کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2561366 | 1126904 | 2010 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Quercetin attenuates Monocyte Chemoattractant Protein-1 gene expression in glucose primed aortic endothelial cells through NF-κB and AP-1
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کلمات کلیدی
NF-κBRAECsTRITCPKCAP-1DAPIMCP-1DCFH-DART-PCRHUVECSNACFITC2′,7′-dichlorofluorescein diacetate - 2 '، 7'-dichlorofluorescein diacetate4′,6-diamidino-2-phenylindole - 4 '، 6-دیامیدینو-2-فنیلینولMAPK - MAPKMitogen activated protein kinases - Mitogen فعال پروتئین کینازROS - ROSElectrophoretic mobility shift assay - آزمون تحرک تحرک الکتروفورزstandard deviation - انحراف معیارEnzyme-linked immunosorbent assay - تست الیزاELISA - تست الیزاRat aortic endothelial cells - سلول اندوتلیال آئورت موش صحراییHuman umbilical vein endothelial cells - سلول های اندوتلیالی ورید ناف انسانEndothelial cells - سلولهای اندوتلیالEMSA یا electrophoretic mobility shift assay - سنجش تغییر تحرک الکتروفورتیکnuclear factor-κB - فاکتور هسته ای κBfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتN-acetyl cysteine - نیتستیل سیستئینReverse transcriptase-polymerase chain reaction - واکنش زنجیره ای واکنش زنجیره ای واکنش زنجیره ایmonocyte chemoattractant protein-1 - پروتئین شیمیایی monocyte chemoattractant-1activator protein-1 - پروتئین فعال کننده-1Protein kinase C - پروتئین کیناز سیQuercetin - کوئرستینGlucose - گلوکزReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
داروشناسی
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![عکس صفحه اول مقاله: Quercetin attenuates Monocyte Chemoattractant Protein-1 gene expression in glucose primed aortic endothelial cells through NF-κB and AP-1 Quercetin attenuates Monocyte Chemoattractant Protein-1 gene expression in glucose primed aortic endothelial cells through NF-κB and AP-1](/preview/png/2561366.png)
چکیده انگلیسی
Monocyte Chemoattractant Protein-1 (MCP-1) is involved in the diapedesis of blood monocytes into the arterial intima, an early critical event in atherogenesis. Modulating MCP-1 expression can be a key strategy to decrease the risk for atherosclerosis in diabetes. We hypothesized that quercetin, an anti-inflammatory molecule could modulate high glucose concentration (HG) induced MCP-1 expression in aortic endothelial cells in vitro because of its regulatory effects on Activator Protein-1 (AP-1) and Nuclear Factor-κB (NF-κB). Rat aortic endothelial cells (RAECs) were exposed to HG in the presence or absence of quercetin. Quercetin attenuated HG induced MCP-1 mRNA (42%) and protein synthesis (45%) when estimated using real-time reverse transcriptase-polymerase chain reaction and enzyme-linked immunosorbent assay respectively. Western blot analysis found quercetin to maintain cytosolic p65 protein levels to that seen in control. Quercetin was found to attenuate HG induced increased NF-κB and AP-1 DNA binding activity in electrophoretic mobility shift assay. Immunofluorescence studies revealed quercetin to prevent HG induced nuclear localization of p65 and c-jun. Quercetin was also found to decrease HG induced activation of NF-κB (71% ± 14%), AP-1 (69% ± 24%) and MCP-1 promoter (79% ± 25%) in EA.hy926 cells when analyzed using luciferase reporter assay.We conclude that quercetin attenuates MCP-1 expression in HG treated RAECs, probably by regulating both NF-κB and AP-1 pathways. The findings provide new insights into HG induced MCP-1 gene regulation in aortic endothelial cells and the potential of quercetin in abating the risk for atherosclerosis in diabetes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Pharmacological Research - Volume 62, Issue 4, October 2010, Pages 328-336
Journal: Pharmacological Research - Volume 62, Issue 4, October 2010, Pages 328-336
نویسندگان
Sumith Retnamma Panicker, Prethish Sreenivas, Mani Sankar Babu, Devarajan Karunagaran, Chandrasekharan Cheranellore Kartha,