کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2581526 | 1130194 | 2009 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A proteomic investigation into the human cervical cancer cell line HeLa treated with dicitratoytterbium (III) complex
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
2′7′-dichlorofluoresceineEF2PHBVDACDTTGAPDHJC-1DCFH-DAPTPdl-dithiothreitolΔΨmPARPSOD13-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide - 3- (4،5-Dimethylthiazol-2-yl) -2،5-diphenyltetrazoliumbromideMTT - MTTROS - ROSPermeability transition pore - افت فشار نفوذ پذیریYtterbium - ایتربیمHeLa cells - سلول های هلاeukaryotic translation elongation factor 2 - ضریب کشش ترجمه یوکاریوتی 2Quinolinate phosphoribosyltransferase - فسفریبوسیل ترانسفراز شریانیProhibitin - ممنوعیتHuman papillomavirus - ویروس پاپیلوم انسانیHPV - ویروس پایپلوم انسانیMitochondrial transmembrane potential - پتانسیل ترانزیتی میتوکندریProteomics - پروتئومیکسpoly ADP-ribose polymerase - پلی ADA-ribose پلیمرازvoltage-dependent anion-selective channel - کانال انتخابی آنیونی وابسته به ولتاژglyceraldehyde 3-phosphate dehydrogenase - گلیسرولیدید 3-فسفات دهیدروژنازReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Lanthanides have been reported to induce apoptosis in cancer cell lines. Human cervical cancer cell line HeLa was found to be more sensitive to dicitratolanthanum (III) complex ([LaCit2]3â) than other cancer cell lines. However, the effect and mechanism of dicitratoytterbium (III) complex ([YbCit2]3â) on HeLa cells is unknown. Using biochemical and comparative proteomic analyses, [YbCit2]3â was found to inhibit HeLa cell growth and induce apoptosis. Similar to the effects of [LaCit2]3â, proteomics results from [YbCit2]3â-treated cells revealed profound changes in proteins relating to mitochondria and oxidative stress, suggesting that mitochondrial dysfunction plays a key role in [YbCit2]3â-induced apoptosis. This was confirmed by the decreased mitochondrial transmembrane potential and the increased generation of reactive oxygen species in [YbCit2]3â-treated cells. Western blot analysis showed that [YbCit2]3â-induced apoptosis was accompanied by the activation of caspase-9 and specific proteolytic cleavage of PARP, leading to an increase in the pro-apoptotic protein Bax and a decrease in the anti-apoptotic protein Bcl-2. These results suggest a mitochondrial pathway of cell apoptosis in [YbCit2]3â-treated cells, which will help us understand the molecular mechanisms of lanthanide-induced apoptosis in tumor cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemico-Biological Interactions - Volume 181, Issue 3, 30 October 2009, Pages 455-462
Journal: Chemico-Biological Interactions - Volume 181, Issue 3, 30 October 2009, Pages 455-462
نویسندگان
Liming Shen, Qiong Liu, Jiazuan Ni, Guangyan Hong,