کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2581526 | 1130194 | 2009 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A proteomic investigation into the human cervical cancer cell line HeLa treated with dicitratoytterbium (III) complex
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کلمات کلیدی
2′7′-dichlorofluoresceineEF2PHBVDACDTTGAPDHJC-1DCFH-DAPTPdl-dithiothreitolΔΨmPARPSOD13-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide - 3- (4،5-Dimethylthiazol-2-yl) -2،5-diphenyltetrazoliumbromideMTT - MTTROS - ROSPermeability transition pore - افت فشار نفوذ پذیریYtterbium - ایتربیمHeLa cells - سلول های هلاeukaryotic translation elongation factor 2 - ضریب کشش ترجمه یوکاریوتی 2Quinolinate phosphoribosyltransferase - فسفریبوسیل ترانسفراز شریانیProhibitin - ممنوعیتHuman papillomavirus - ویروس پاپیلوم انسانیHPV - ویروس پایپلوم انسانیMitochondrial transmembrane potential - پتانسیل ترانزیتی میتوکندریProteomics - پروتئومیکسpoly ADP-ribose polymerase - پلی ADA-ribose پلیمرازvoltage-dependent anion-selective channel - کانال انتخابی آنیونی وابسته به ولتاژglyceraldehyde 3-phosphate dehydrogenase - گلیسرولیدید 3-فسفات دهیدروژنازReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A proteomic investigation into the human cervical cancer cell line HeLa treated with dicitratoytterbium (III) complex A proteomic investigation into the human cervical cancer cell line HeLa treated with dicitratoytterbium (III) complex](/preview/png/2581526.png)
چکیده انگلیسی
Lanthanides have been reported to induce apoptosis in cancer cell lines. Human cervical cancer cell line HeLa was found to be more sensitive to dicitratolanthanum (III) complex ([LaCit2]3â) than other cancer cell lines. However, the effect and mechanism of dicitratoytterbium (III) complex ([YbCit2]3â) on HeLa cells is unknown. Using biochemical and comparative proteomic analyses, [YbCit2]3â was found to inhibit HeLa cell growth and induce apoptosis. Similar to the effects of [LaCit2]3â, proteomics results from [YbCit2]3â-treated cells revealed profound changes in proteins relating to mitochondria and oxidative stress, suggesting that mitochondrial dysfunction plays a key role in [YbCit2]3â-induced apoptosis. This was confirmed by the decreased mitochondrial transmembrane potential and the increased generation of reactive oxygen species in [YbCit2]3â-treated cells. Western blot analysis showed that [YbCit2]3â-induced apoptosis was accompanied by the activation of caspase-9 and specific proteolytic cleavage of PARP, leading to an increase in the pro-apoptotic protein Bax and a decrease in the anti-apoptotic protein Bcl-2. These results suggest a mitochondrial pathway of cell apoptosis in [YbCit2]3â-treated cells, which will help us understand the molecular mechanisms of lanthanide-induced apoptosis in tumor cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemico-Biological Interactions - Volume 181, Issue 3, 30 October 2009, Pages 455-462
Journal: Chemico-Biological Interactions - Volume 181, Issue 3, 30 October 2009, Pages 455-462
نویسندگان
Liming Shen, Qiong Liu, Jiazuan Ni, Guangyan Hong,