کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2603297 | 1133816 | 2007 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Application of a yeast estrogen screen in non-biomarker species Varicorhinus barbatulus fish with two estrogen receptor subtypes to assess xenoestrogens
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کلمات کلیدی
BPADDEdi-n-butyl phthalate4-NonylphenolEREPCBsNCBIXenoestrogenONPG1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene5′-Rapid amplification of cDNA ends17β-estradiol - 17β استرادیول4-t-Octylphenol - 4-تی-اکتیلفنول5′-RACE - 5'-RACEDMSO - DMSOE1, Estrone - E1، استرنEDCs - EDC ها4-NP - NP-4YES - بلهBenzyl butyl phthalate - بنزیل بوتیل فتالاتBisphenol A - بیسفنول ای، بیسفنول APolychlorinated biphenyls - بیفنیل پلیکلر synthetic dropout - ترک خوردگی مصنوعیBBP - تولید ناخالص داخلیDDT - دیکرو دیفنیل تری کلرواتانDimethyl sulfoxide - دیمتیل سولفواکسیدYeast Estrogen Screen - صفحه نمایش استروئید مخمرestrogen response element - عنصر پاسخ استروژنTranscriptional activation - فعال سازی رونویسیThe National Center for Biotechnology Information - مرکز ملی بیوتکنولوژی اطلاعاتEndocrine Disrupting Chemicals - مواد شیمیایی خرابکار غدد درون ریزpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازoptical density - چگالی نوریLigand efficiency - کارایی لیگاندEstrogen receptor - گیرنده استروژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Application of a yeast estrogen screen in non-biomarker species Varicorhinus barbatulus fish with two estrogen receptor subtypes to assess xenoestrogens Application of a yeast estrogen screen in non-biomarker species Varicorhinus barbatulus fish with two estrogen receptor subtypes to assess xenoestrogens](/preview/png/2603297.png)
چکیده انگلیسی
Xenoestrogens can interfere with normal estrogen signaling by competitively binding to the estrogen receptor (ER) and activating transcription of target genes. In this study, we cloned the estrogen receptor alpha (vbERα) and beta 2 (vbERβ2) genes from liver of the indigenous Taiwanese cyprinid fish Varicorhinus barbatulus and tested the direct impact of several xenoestrogens on these ERs. Transcriptional activity of xenoestrogens was measured by the enzymatic activity of estrogen responsive element (ERE)-containing β-galactosidase in a yeast reporter system. The xenoestrogens tested were phenol derivatives, DDT-related substances, phthalic acid esters, and polychlorinated biphenyls, with 17β-estradiol (E2) as a subjective standard. The phenol derivatives [4-nonylphenol (4-NP), 4-t-octylphenol (4-t-OP) and bisphenol A (BPA)] exhibited significant dose-dependent responses in both ligand potency and ligand efficiency. Consistent with yeast assays using human or rainbow trout ERs, we observed a general subtype preference in that vbERα displayed higher relative potencies and efficiencies than vbERβ2, although our assays induced a stronger response for xenoestrogens than did human or trout ERs. Whereas 4-NP and 4-t-OP have similar EC50 values relative to E2 for both ER subtypes, the strong estrogenic response of BPA markedly differentiates vbERα from vbERβ2, suggesting possible species-specific BPA sensitivity. We report that the ameliorative yeast tool is readily applicable for indigenous wildlife studies of the bio-toxic influence of xenoestrogens with wildlife-specific estrogen receptors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 21, Issue 4, June 2007, Pages 604-612
Journal: Toxicology in Vitro - Volume 21, Issue 4, June 2007, Pages 604-612
نویسندگان
Keng-Yen Fu, Chung-Yuan Chen, Whei-meih Chang,