کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
30304 | 44468 | 2014 | 7 صفحه PDF | دانلود رایگان |
• Two Types of Cl−/Br−/I− substituted PSII have been prepared and investigated.
• Remarkable changes of OEC are observed only in Type 2 preparations.
• The high-affinity Cl− plays key roles for water oxidation in PSII.
• The location of high-affinity Cl− may be different to those revealed in X-ray data.
The high-affinity chloride ion (Cl−) is known to play a key role in water oxidation in photosystem II (PSII). Recent crystallographic studies revealed two Cl− binding sites in PSII. To examine whether these two Cl− ions are correlated to the high-affinity Cl−, we prepared Cl−/Br−/I−-substituted PSII samples from both higher plants and cyanobacteria by using two different protocols: one was the method used in the crystallographic study (Type 1) and the other was a method developed recently to ensure the efficient replacement of Cl− (Type 2). While only minor effects were observed in the Type 1 preparation, efficient Br−/I−substitution by the Type 2 protocol led to significant changes in the EPR properties of the oxygen-evolving complex (OEC) and the TyrZ, as well as in oxygen-evolving activities. These results are discussed in terms of the binding site of the high-affinity Cl− relative to the two Cl− ions revealed by the recent X-ray structural data.
The high-affinity Cl− is known to play roles for function of PSII. Two types of Cl−/Br−/I− substituted PSII samples were prepared to probe its location and function. Significant changes of the OEC was observed only in preparations with efficient Br−/I−substitution, but not in the method used in recent crystallographic study. These results provide new insights to understanding the function and location of the high-affinity Cl− in PSII.Figure optionsDownload as PowerPoint slide
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 138, 5 September 2014, Pages 249–255