کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
30789 | 44503 | 2012 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: The effect of Se salts on DNA structure The effect of Se salts on DNA structure](/preview/png/30789.png)
There is considerable interest in the role of selenium in cancer prevention. Various organic and inorganic Se compounds are considered to be antioxidants. In the present study, the binding modes, the binding constants and the stability of Se–DNA complexes have been determined by Fourier transform infrared (FTIR) and UV–Visible spectroscopic methods. Spectroscopic evidence showed that Na2SeO4 and Na2SeO3 bind to the minor and major grooves of DNA and the backbone phosphate (PO2) with overall binding constants of K(Na2SeO4–DNA) = 5.20 × 104 M−1 and K(Na2SeO3–DNA) = 1.87 × 103 M−1. DNA aggregations occurred at high selenium concentrations. No biopolymer conformational changes were observed upon Na2SeO3 and Na2SeO4 interactions, while DNA remained in the B-family structure.
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► The binding affinity of Na2SeO4 and Na2SeO3 to DNA was determined.
► The binding constant of Na2SeO4–DNA complexes is higher than Na2SeO3–DNA complexes.
► Na2SeO4 and Na2SeO3 mainly interact with G, A, T and backbone PO2 group.
► DNA aggregation occurred at higher selenium salts concentrations.
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 113, 1 August 2012, Pages 36–41