کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
30822 44506 2006 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Steady-state fluorescence quenching applications for studying protein structure and dynamics
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Steady-state fluorescence quenching applications for studying protein structure and dynamics
چکیده انگلیسی

Fluorescence quenching methods are useful to obtain information about the conformational and/or dynamic changes of proteins in complex macromolecular systems. In this review steady-state methods are described and the data interpretation is thoroughly discussed. As a special case of fluorescence quenching mechanism, fluorescence resonance energy transfer (FRET) phenomenon is also presented. Application of a FRET based method to characterize the temperature dependence of the flexibility of protein matrix is clearly demonstrated.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 83, Issue 3, 1 June 2006, Pages 223–236
نویسندگان
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