کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3199842 | 1201922 | 2011 | 8 صفحه PDF | دانلود رایگان |

BackgroundThe hygiene hypothesis suggests that parasitic infection modulates host immune responses and decreases atopy. Other data suggest parasitic infections may induce allergic responsiveness.ObjectiveTo assess the structural and immunologic relationships between the known Dermatophagoides pteronyssinus (Der p 10) tropomyosin allergen and filarial tropomyosin of Onchocerca volvulus (OvTrop).MethodsThe molecular, structural, and immunologic relationships between OvTrop and Der p 10 were compared. Levels of OvTrop-specific and Der p 10–specific IgE, IgG, and IgG4 in sera of filaria-infected and filarial-uninfected D pteronyssinus–atopic individuals were compared, as were the responses in nonhuman primates infected with the filarial parasite Loa loa. Cross-reactivity was compared by antigen-mediated depletion assays and functionality by passive basophil sensitization.ResultsFilarial and mite tropomyosins were very similar, with 72% identity at the amino acid level, and overlapping predicted 3-dimensional structures. The prevalence of IgE and IgG to Der p 10 was increased in filaria-infected individuals compared with uninfected subjects. There was a strong correlation between serum levels of Ov- and Der p 10–tropomyosin—specific IgE, IgG, and IgG4 (P < .0001; r > 0.79). Preincubation of sera from anti–Der p 10—positive subjects with OvTrop completely depleted IgE, IgG, and IgG4 anti–Der p 10. Basophils sensitized with sera from individuals allergic to Der p 10 released histamine similarly when triggered with OvTrop or Der p 10. Primates experimentally infected with L loa developed IgE that cross-reacted with Der p 10.ConclusionFilarial infection induces strong cross-reactive antitropomyosin antibody responses that may affect sensitization and regulation of allergic reactivity.
Journal: Journal of Allergy and Clinical Immunology - Volume 127, Issue 2, February 2011, Pages 479–486