کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3199964 1201924 2010 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites
چکیده انگلیسی

BackgroundDendritic cells (DCs) regulate the immune response to allergens in the lung; they induce either effector or regulatory T cells, which promote or suppress, respectively, the development of allergy. IL-10 is a potent immunosuppressive cytokine that induces type 1 regulatory (Tr1) T cells.ObjectiveTo generate allergen-specific Tr1 cells in vitro from children with allergy.MethodsMonocyte-derived DCs from children with allergy to house dust mites (HDM) were generated by incubating the cells with IL-10 and pulsing them with Der p 2, a major HDM allergen, or by pulsing them with Der p 2 and incubating them with IL-10 during their last 2 days of differentiation.ResultsDer p 2–specific T-cell proliferation and TH2 cytokine production were significantly reduced when T cells from patients with allergy to HDM were activated with autologous Der p 2–pulsed DCs that had been differentiated or incubated with IL-10. T-cell lines generated with Der p 2–pulsed DCs that were differentiated with IL-10 were hyporesponsive to reactivation with Der p 2 and able to suppress Der p 2–specific TH2 effector cells.ConclusionDendritic cells differentiated in the presence of IL-10 and pulsed with allergen gave rise to a population of tolerogenic DCs that induced allergen-specific Tr1 cells. This finding represents an important step forward to the prospective clinical application of tolerogenic DCs to modulate allergen-specific T-cell responses.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Allergy and Clinical Immunology - Volume 125, Issue 3, March 2010, Pages 727–736
نویسندگان
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