کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3202143 1201967 2007 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Exposure to a farming environment has allergen-specific protective effects on TH2-dependent isotype switching in response to common inhalants
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Exposure to a farming environment has allergen-specific protective effects on TH2-dependent isotype switching in response to common inhalants
چکیده انگلیسی

BackgroundIgE synthesis by human B cells results from allergen-dependent, TH2-mediated isotype switching. Exposure to a farming environment protects against IgE responses.ObjectiveWe reconstructed allergen-dependent switching patterns in vivo to identify the level or levels at which farm exposure acts to protect against atopy.MethodsSerum IgG1 to IgG4 and IgE to grass (rPhl p 1 and rPhl p 5), cat (rFel d 1), and mite (rDer p 2) were assessed by means of ELISA in the Allergy and Endotoxin study population (812 children). Farm exposure was defined as currently living on a farm, exposure to stables/farm milk in the first year of life, or both.ResultsFarm exposure did not affect allergen-specific IgG2 and IgG3 levels but had complex allergen-specific effects on IgG1, IgG4, and IgE levels. Exposure protected against grass-specific responses at every step along the IgG1/IgG4/IgE switching pathway but had no significant effect on mite responses. Protection from cat responses was concentrated at the IgG1 level. For all allergens, failure to express IgG1 was associated with low prevalence of IgG4 or IgE responses. Notably, coexpression of IgG1, IgG4, and IgE to grass was associated with increased risk of allergic disease and higher IgE levels compared with production of IgG1 and IgE without IgG4, suggesting IgG4 coexpression marks stronger activation of TH2-dependent events.ConclusionThe protective effects of farm exposure were confined to TH2-dependent IgG1, IgG4, and IgE expression and were allergen and switch stage specific, suggesting that distinct mechanisms regulate individual steps within allergen-induced class switching in vivo.Clinical implicationsEnvironmental interventions to prevent IgE expression might need to be tailored to specific allergens.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Allergy and Clinical Immunology - Volume 119, Issue 2, February 2007, Pages 351–358
نویسندگان
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