کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3203704 1202010 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Biological material on inhaled coarse fraction particulate matter activates airway phagocytes in vivo in healthy volunteers
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Biological material on inhaled coarse fraction particulate matter activates airway phagocytes in vivo in healthy volunteers
چکیده انگلیسی

BackgroundIn vitro, endotoxin on coarse fraction particulate matter (PM2.5-10) accounts for the majority of the ability of PM2.5-10 to induce cytokine responses from alveolar macrophages.ObjectiveWe examined in vivo whether inhaled PM2.5-10 from local ambient air induce inflammatory and immune responses in the airways of healthy human beings and whether biologic material on PM2.5-10 accounts for these effects.MethodsOn 3 separate visits, 9 healthy subjects inhaled nebulized saline (0.9%, control), PM2.5-10 collected from local ambient air that was heated to inactivate biological material (PM2.5-10−), or nonheated PM (PM2.5-10+). PM2.5-10 deposition (∼0.65 mg/subject) targeted the bronchial airways (confirmed by using radiolabeled aerosol), and induced sputum was obtained 2 to 3 hours postinhalation for analysis of cellular and biochemical markers of inflammation and innate immune function.ResultsInhaled PM2.5-10+ induced elevated inflammation (% PMNs, macrophage mRNA TNF-α), increased eotaxin, upregulated immune surface phenotypes on macrophages (mCD14, CD11b, HLA-DR), and increased phagocytosis (monocytes) versus saline (P < .05). Biological inactivation of PM2.5-10 (PM2.5-10−) had no effect on neutrophilia but significantly (P < .05) attenuated mRNA TNF-α, eotaxin levels, cell surface marker responses, and phagocytosis.ConclusionBiological components of PM2.5-10 are not necessary to induce neutrophil responses but are essential in mediating macrophage responses. The ability of PM2.5-10 to activate monocytic cells and potentially skew the airways toward an allergic phenotype by enhancing eotaxin levels may enhance responses to allergens or bacteria in individuals with allergy.Clinical implicationsPM2.5-10 might enhance the response of individuals with allergy to airborne bacteria.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Allergy and Clinical Immunology - Volume 117, Issue 6, June 2006, Pages 1396–1403
نویسندگان
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