The effects of bromelain on angiogenesis, nitric oxide, and matrix metalloproteinase-3 and -9 in rats exposed to electrical burn injury

This study was aimed to investigate the effects of bromelain on angiogenesis, nitric oxide, and matrix metalloproteinase-3 and -9 in rats exposed to 1200 mV electrical burn injury. Thirty-five, male Wistar albino rats was divided into five groups (n = 7 each), including control (untreated) group; electrical burn injury (EI) group; electrical burn injury + 21.25 mg/kg BW bromelain group (EIB1); electrical burn injury + 42.50 mg/kg BW bromelain group (EIB2); and electrical burn injury + 85.00 mg/kg BW bromelain group (EIB3). Rat models of electrical burns done by providing electricity in rats that had been anesthetized, a voltage of 1200 mV and strong currents 15 mA for 10 s. The VEFG, NO, and MMP-9 levels were significantly greater in the EI group compared to the untreated group. Out of the 21.25 mg/kg BW, 42.50 mg/kg BW, and 85 mg/kg BW doses of Bromelain extract, only the lowest doses prevented EI-induced increase in NO and MMP-9 level (P < 0.05). Bromelain at the lowest dose (21.25 mg/kg BW) act as anti-inflammatory and modulate the matrix metalloproteinase-9 in rats exposed to 1200 mV electrical burn injury.