کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3415827 | 1593658 | 2006 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Co-administration of plasmid expressing IL-12 with 14-kDa Schistosoma mansoni fatty acid-binding protein cDNA alters immune response profiles and fails to enhance protection induced by Sm14 DNA vaccine alone
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کلمات کلیدی
ADCCCD8IgG2aIFN-γSm14IL-10BALCD4PBSTIL-4IL-12FITCIgGFBSLPSPBScDNA - cDNADNA - DNA یا اسید دزوکسی ریبونوکلئیکIgG1 - IgG1 وo-Phenylenediamine - o-phenylenediamineopd - UPDdeoxyribonucleic acid - اسید deoxyribonucleicCon A - با ABreg - برگیNatural killer cells - سلولهای کشنده طبیعیAntibody-dependent cell cytotoxicity - سمیت سلولی وابسته به آنتی بادیSchistosoma mansoni - شیستوزومای مانسونیTNF-α - فاکتور نکروز توموری آلفاDNA vaccine - واکسن DNA
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Schistosomiasis is an endemic disease that affects 200 million people worldwide. DNA-based vaccine is a promising strategy to induce protective immunity against schistosomiasis, since both humoral and cellular immune responses are involved in parasite elimination. In this study, we evaluated the ability of Sm14 cDNA alone or in association with a plasmid expressing murine interleukin (IL)-12 to induce protection against challenge infection. Mice were immunized with four doses of the DNA vaccine and the levels of protection were determined by worm burden recovery after challenge infection. Specific antibody production to rSm14 was determined by ELISA, and cytokine production was measured in splenocyte culture supernatants stimulated with rSm14 and in bronchoalveolar lavage of vaccinated mice after challenge infection. DNA immunization with pCI/Sm14 alone induced 40.5% of worm reduction. However, the use of pCI/IL-12 as adjuvant to pCI/Sm14 immunization failed to enhance protection against challenge infection. Protection induced by pCI/Sm14 immunization correlates with specific IgG antibody production against Sm14, Th1 type of immune response with high levels of interferon (IFN)-γ and low levels of IL-4 in splenocyte culture supernatants and in brochoalveolar lavage after challenge infection. IL-12 co-administration with pCI/Sm14 induced a significant production of nitric oxide in splenocyte culture supernatants and also lymphocyte suppression, with reduced percentage of T cells producing IFN-γ and tumor necrosis factor-α.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbes and Infection - Volume 8, Issues 9â10, August 2006, Pages 2509-2516
Journal: Microbes and Infection - Volume 8, Issues 9â10, August 2006, Pages 2509-2516
نویسندگان
Cristina T. Fonseca, Lucila G.G. PacÃfico, Michele M. Barsante, Tatiana Rassi, Geovanni D. Cassali, Sérgio. C. Oliveira,