کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4341794 | 1295846 | 2008 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Activation of microglia and p38 mitogen-activated protein kinase in the dorsal column nucleus contributes to tactile allodynia following peripheral nerve injury
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کلمات کلیدی
p-p38PWTSNLphosphorylated p38SB203580Tissue necrosis factor-αiNOSERKCOX-2GFAPJnkPLSDPWLABCNeuNc-Jun N-terminal kinase - C-Jun N-terminal kinaseMAPK - MAPKpaw withdrawal threshold - آستانه برداشتن پاimmunoreactive - ایمنی فعالImmunohistochemistry - ایمونوهیستوشیمیpaw withdrawal latency - تاخیر در برداشت پنجهanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of varianceNeuropathic pain - درد نوروپاتیکBehavior - رفتارinducible nitric oxide synthase - سنتاز اکسید نیتریک القاییCyclooxygenase-2 - سیکلوکوکسیژناز2Spinal cord - طناب نخاعیTNF-α - فاکتور نکروز توموری آلفاgracile nucleus - هسته شادneuronal nuclei - هسته های نورونیGlial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالmitogen-activated protein kinase - پروتئین کیناز فعال با mitogenspinal nerve ligation - پیوند عصب ستون فقراتavidin–biotin complex - پیچیده avidin-بیوتینextracellular signal-regulated kinase - کیناز تنظیم شده سیگنال خارج سلولی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The activation of glial cells in the CNS has been suggested to be involved in abnormal pain sensation after peripheral nerve injury. Previous studies demonstrated phosphorylation of p38 mitogen-activated protein kinase (MAPK) in spinal cord glial cells after peripheral nerve injury, and such phosphorylation has been suggested to be involved in the development of neuropathic pain. The aim of this study was to examine the dorsal column nuclei for phosphorylation of p38 MAPK following peripheral nerve injury and to explore a possibility of its contribution to neuropathic pain. Immunohistochemical labeling for phosphorylated p38 (p-p38) MAPK was performed in histological sections of the rat spinal cord and medulla oblongata after the fifth lumbar (L5) spinal nerve ligation (SNL). The number of p-p38 MAPK-immunoreactive (IR) cells was significantly increased in the L5 dorsal horn and the gracile nucleus ipsilateral to the injury at days 3-21 after SNL. Double immunofluorescence labeling with cell-specific markers revealed that p-p38 MAPK-IR cells co-expressed OX-42, suggesting their microglial identity. Increased immunofluorescence labeling for OX-42 indicated that microglial cells were activated by SNL in the L5 dorsal horn and the gracile nucleus ipsilateral to the injury. Continuous infusion of a p38 MAPK inhibitor into the cisterna magna for 14 days beginning on the day of SNL suppressed the development of tactile allodynia, but not thermal hyperalgesia induced by nerve injury. These results demonstrate that SNL activates p38 MAPK pathway in microglia in the gracile nucleus as well as in the spinal cord dorsal horn. Activation of p38 MAPK in medullary microglia may contribute to the pathogenesis of neuropathic pain.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 153, Issue 4, 2 June 2008, Pages 1245-1255
Journal: Neuroscience - Volume 153, Issue 4, 2 June 2008, Pages 1245-1255
نویسندگان
R. Terayama, S. Omura, N. Fujisawa, T. Yamaai, H. Ichikawa, T. Sugimoto,