کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4477056 1622769 2008 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characteristics of ChgH–GFP transgenic medaka lines, an in vivo estrogenic compound detection system
موضوعات مرتبط
مهندسی و علوم پایه علوم زمین و سیارات اقیانوس شناسی
پیش نمایش صفحه اول مقاله
Characteristics of ChgH–GFP transgenic medaka lines, an in vivo estrogenic compound detection system
چکیده انگلیسی

We previously reported the characteristics of a ChgH–GFP transgenic medaka line that indicates estrogenic compound pollution in environmental water by the green fluorescence of their liver. Recently, we established four more lines. In this study, the characteristics of the five transgenic medaka lines were investigated. The intensity of reporter gene expression varied among transgenic lines and generally correlated well with the amount of integrated transgene in each line. Line-specific ectopic expression was also observed. However, the sensitivity to 17-β estradiol did not differ among transgenic lines. Three transgenic lines are considered to be suitable as bio-indicators of estrogenic activity, due to the ease of observing green fluorescence in their livers. The transgenic lines can also detect the estrogenic activity of testosterone and 17-β trenbolone at the nominal concentration of 30 and 100 μg/l, respectively.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Marine Pollution Bulletin - Volume 57, Issues 6–12, 2008, Pages 441–444
نویسندگان
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