Extraction, enumeration and identification methods for monitoring microplastics in the environment

• We summarized the basic techniques and easily omitted factors in the extraction from sediment, seawater and organism.
• The impact of microscopies on the analysis of enumeration can not be negligible.
• Advantages and challenges of using micro-FTIR, art-FTIR, Pry-GC/MS, Raman spectroscopy and EDS are discussed.

There is much research on the occurrence, pollution characteristics and impacts of microplastics in the marine environment but this omits factors which play important roles in the analysis of microplastics. This review summarizes the methods and techniques in the extraction from sediment, seawater and organisms, and assesses their advantages and limitations according to different experimental conditions, such as salt solution and reagents added to remove organic matter. Similarly, this overview includes the enumeration methods of microplastics by many kinds of microscopes (e.g. stereomicroscope, fluorescent microscope, scanning electron microscope). Advantages and challenges of using micro-FTIR, ART-FTIR, FPA-FTIR, Pry-GC/MS, and Raman spectroscopy in the identification methods are also discussed. This review suggests that monitoring microplastics needs standardized protocols for extraction, identification and quantification and that further research on the effects of microplastics to human health is needed.

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