Antioxidant and pro-oxidant challenge of tannic acid in mussel hemocytes exposed to cadmium

The present study investigates the antioxidant and pro-oxidant behavior of tannic acid (TA) in hemocytes of mussel Mytilus galloprovincialis, in the presence or the absence of cadmium (Cd). TA at concentrations up to 20 μM, primarily found to be no toxic (in terms of cell viability, superoxide anions, nitric oxide and lipid peroxidation products currently estimated), significantly diminished the cytotoxic and oxidative effects induced by the metal (50 and/or 100 μM) in all cases. On the other hand, higher concentrations of TA (40 and 60 μM) were toxic, thus enhancing Cd-mediated cytotoxic and oxidative effects. The present study showed TA beneficiary properties in hemocytes of mussels, at least at low concentrations, while TA at concentrations higher than 20 μM could serve as an excellent oxidized substrate, thus enhancing toxic effects either alone or with the presence of micromolar concentrations of non transition metals, such as Cd.

► Low doses of tannic acid (TA) provide antioxidant and cytoprotective effects.
► Cadmium-induced oxidative effects were diminished in the presence of low doses of TA.
► TA higher than 20 μM acts as pro-oxidant in hemocytes of mussels.
► High concentrations of TA further enhance cadmium-mediated toxic effects.