کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4551322 1328290 2010 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of multi-drug resistance associated proteins MRP1 (ABCC1) and MRP3 (ABCC3) from rainbow trout (Oncorhynchus mykiss)
موضوعات مرتبط
مهندسی و علوم پایه علوم زمین و سیارات اقیانوس شناسی
پیش نمایش صفحه اول مقاله
Identification of multi-drug resistance associated proteins MRP1 (ABCC1) and MRP3 (ABCC3) from rainbow trout (Oncorhynchus mykiss)
چکیده انگلیسی

The MRPs (multi-drug resistance associated proteins; ABCC subfamily) are members of the ATP binding cassette (ABC) superfamily of transport proteins and act as cellular efflux transporters of a wide variety of substrates, in particular glutathione, glucuronide and sulphate conjugates of diverse compounds. Together with P-gp (P-glycoprotein; MDR1; ABCB1) and BCRP (breast cancer resistance protein, ABCG2) the MRPs are highly important as cellular defense against toxicants and confer multixenobiotic resistance (MXR). In aquatic ecotoxicology MXR research has mostly focussed on P-glycoprotein while the other relevant ABC transporters are widely under-appreciated. We here present complete MRP1 (ABCC1) and partial MRP3 (ABCC3) cDNAs from rainbow trout (Oncorhynchus mykiss). We identified 4398 bp of the MRP1 and 3786 bp of the MRP3 open reading frames (ORF) by screening the NCBI EST database and by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) using RNA from RTgill-W1 cells. Identities with human homologs are 56% for MRP1 and 64% for MRP3.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Marine Environmental Research - Volume 69, Supplement 1, 2010, Pages S7–S10
نویسندگان
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