کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4551719 1328319 2006 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The sea urchin embryo as a model for studying efflux transporters: Roles and energy cost
موضوعات مرتبط
مهندسی و علوم پایه علوم زمین و سیارات اقیانوس شناسی
پیش نمایش صفحه اول مقاله
The sea urchin embryo as a model for studying efflux transporters: Roles and energy cost
چکیده انگلیسی

We describe the use of the sea urchin as a model for studying efflux transporters and estimating energy cost for the cytotoxin protective system provided by these transporters. The unfertilized egg has low transport activity, which increases to a new steady state shortly after fertilization. Activity results from p-glycoprotein (p-gp) and MRP type transporters which protect the embryo from cytotoxic drugs that can disrupt cell division or induce apoptosis. The energy cost is estimated from a novel use of calcein-AM as a substrate; keeping 0.25 μM substrate levels out of the cell utilizes only 0.023% of steady state respiration.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Marine Environmental Research - Volume 62, Supplement 1, 2006, Pages S1–S4
نویسندگان
, , , ,