کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5452902 | 1513833 | 2017 | 24 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cold atmospheric plasma treatment affects early bacterial adhesion and decontamination of soft reline palatal obturators
ترجمه فارسی عنوان
درمان پلاسما در محیط سرد بر روی چسبندگی زودهنگام باکتری ها و تخریب زدایی اپورتاتورهای نرم افزاری پالکتیو تاثیر می گذارد
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی مواد
دانش مواد (عمومی)
چکیده انگلیسی
The aim of this work was to evaluate cold atmospheric plasma bactericide activity towards biofilm formation on soft reline oral palatal obturators. Plasma was generated using two dielectric barrier discharge and tested against the oral biofilm formers Streptococcus mutans and Aggregatibacter actinomycetemcomitans. Relines were either plasma treated before being infected with 24Â h biofilm, or infected with 90Â min (early) or 24Â h (mature) biofilm prior to be plasma treated for 30, 60, or 120Â s. Bacteria numbers and viability were evaluated by Colonies Forming Units counts (CFU) and quantitative colorimetric metabolic 2,3-bis (2-methoxy-4-nitro-5-sulphophenyl)-5-[(phenyl amino) carbonyl]-2H-tetrazolium hydroxide assay (XTT). cytocompatibility was assayed towards human primary fibroblasts and keratinocytes cultivated on plasma-treated specimens by colorimetric metabolic 3-(4,5-Dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide (MTT) assay and light microscopy. Results demonstrated that both plasma sources were effective. Plasma treatment of sterile reline affected bacterial adhesion, reducing CFU number and viability by 2 logs and by 50%, in comparison with untreated controls (p < 0.05). Plasma efficacy was marked against early biofilm: after 120Â s, CFUs were reduced by 3 logs, and viability decreased by about 60% (p < 0.05). Significant results were also observed for mature biofilm: CFUs and viability were reduced by approximately 2 logs and 40% (p < 0.05), after 120Â s exposure. Lastly, MTT did not show any toxic effect on human cells. Thus, plasma could represent a very promising skill for soft reline shutters decontamination.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinical Plasma Medicine - Volumes 7â8, December 2017, Pages 36-45
Journal: Clinical Plasma Medicine - Volumes 7â8, December 2017, Pages 36-45
نویسندگان
Anna Liguori, Andrea Cochis, Augusto Stancampiano, Romolo Laurita, Barbara Azzimonti, Rita Sorrentino, Elena Maria Varoni, Marta Petri, Vittorio Colombo, Matteo Gherardi, Lia Rimondini,