کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5500996 | 1534618 | 2017 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
5-Lipoxygenase in monocytes emerges as a therapeutic target for intimal hyperplasia in a murine wire-injured femoral artery
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کلمات کلیدی
α-SMALDPI5-LOVSMCBMDMHMGB1DAPIDAMPPBMCMMP4′6-diamidino-2-phenylindole - 4'6-diamidino-2-phenylindole5-Lipoxygenase - 5-لیپوکسیژنازH&E - H & EROS - ROSAlpha-smooth muscle actin - آکتا عضله آلفا صافdamage-associated molecular patterns - الگوهای مولکولی مرتبط با آسیبVascular remodeling - بازسازی عروقFlap - برآافزا Laser Doppler perfusion imaging - تصویربرداری پرفیوژن لیزر داپلرrestenosis - تنفسPeripheral blood mononuclear cell - سلول تک هسته ای خون محیطیVascular smooth muscle cell - سلول عضله صاف عروقیLeukotriene - لکوترینMatrix metalloproteinases - متالوپروتئیناز ماتریکسwild-type - نوع وحشیHematoxylin and Eosin - هماتوکسیلین و ائوزینHigh mobility group box 1 protein - پروتئین جعبه 1 پروتئین گروه تحرک5-Lipoxygenase activating protein - پروتئین فعال 5-لیپوکسینازReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: 5-Lipoxygenase in monocytes emerges as a therapeutic target for intimal hyperplasia in a murine wire-injured femoral artery 5-Lipoxygenase in monocytes emerges as a therapeutic target for intimal hyperplasia in a murine wire-injured femoral artery](/preview/png/5500996.png)
چکیده انگلیسی
Given the importance of leukotrienes in vascular inflammation induced by local tissue injury, this study investigated the role for 5-lipoxygenase (5-LO) in monocytes in the development of intimal hyperplasia. As a mechanistic study, the importance of monocyte 5-LO in monocyte-macrophage differentiation with subsequent infiltration in neointima was evaluated. In a mouse model of wire-injured femoral artery, intimal hyperplasia started as early as 2Â wks after injury, and luminal area and blood flow were reduced due to increased neointima formation. Time-dependent increases in macrophage infiltration were observed in neointima and showed a positive relationship with neointima volume. In 5-LO-deficient (KO) mice or wild-type (WT) mice treated with an inhibitor of 5-LO activating protein (MK886, 1 and 10Â mg/kg), intimal hyperplasia and macrophage infiltration into neointima were reduced, but monocyte adhesion to injured luminal surface was not inhibited, which suggested 5-LO participates in monocyte-macrophage differentiation. In an in vitro study, monocyte-macrophage differentiation was found to be increased by high mobility group box 1 protein (HMGB1), but this effect was attenuated in cells isolated from 5-LO-KO mice. Furthermore, macrophage infiltration and intimal hyperplasia were more prominent in 5-LO-KO mice transplanted with monocytes from WT mice than in 5-LO-KO mice transplanted with monocytes from 5-LO-KO mice. Taken together, it was suggested that 5-LO in monocytes played a pivotal role in monocyte-macrophage differentiation and subsequent infiltration of macrophage in neointima, leading to vascular remodeling after vascular injury.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease - Volume 1863, Issue 9, September 2017, Pages 2210-2219
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease - Volume 1863, Issue 9, September 2017, Pages 2210-2219
نویسندگان
Seung Eun Baek, Min A. Jang, Seung Jin Lee, So Youn Park, Sun Sik Bae, Chi Dae Kim,