کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5553647 1557957 2017 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of bioactive constituents from the gum resin of Gardenia lucida and its pharmacological potential
ترجمه فارسی عنوان
مشخص کردن اجزای فعال زیست فعال از رزین آدامس گندایا لوسیده و پتانسیل دارویی آن
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی تومور شناسی
چکیده انگلیسی


- Seven polymethoxyflavones were characterized from the gum resin of G. lucida.
- Xanthomicrol exhibited high DPPH and ferric reducing potential.
- Acerosin was found to exhibit broad spectrum anti-proliferative activity.
- 5-Desmethylnobiletin was observed as an effective inhibitor of ODC and CATD.

In the present study we mined the information on Gardenia lucida (Dikamali) and identified seven polymethoxyflavones from its gum resin. We also investigated its antiproliferative and antioxidant potential. Xanthomicrol (8) found as potent DPPH scavenger (85.86 ± 1.3%) along with strong ferric plummeting ability (53.60 ± 2.0 FSE) and reducing potential (1.07 ± 0.01) as compared to ascorbic acid. Gardenin B (5) strongly inhibit biochemical production of nitric oxide (IC50 10.59 ± 0.4 μg/mL) followed by 5-Desmethylnobiletin (7) and Gardenin E (10, IC5011.01 ± 0.7-34.53 ± 2.7 μg/mL). Methanol extract, chloroform fraction and Acerosin (11), Gardenin D (9) and Gardenin B (5) exhibited superior antiproliferative activity against lung, breast, colon, hepatic and leukaemia cell lines as well as in keratinocytes (IC50 12.82 ± 0.67-94.63 ± 1.27 μg/mL) whereas other fractions and isolated compounds moderately affect the cell proliferation (21.40 ± 0.12-48.12 ± 0.47%) with least and non-specific interaction against succinate dehydrogenase. Except compound 2, 3, 6, 8 and 11, others were found as a significant inhibitor of ODC (IC50 2.36 ± 0.7-8.53 ± 0.32 μg/mL) with respect to DFMO (IC50 10.85 ± 0.28 μg/mL). In silico analysis also revealed enervated binding energy (−4.30 to −5.02 kcal/mol) and inhibition constant (704.18-210.26 μM) wherein 5, 7, 8, 9 and 10 showed specific interaction with the receptor while rest were non-specific. Except butanol fraction and Gardenin E, others were potently inhibited the cathepsin D activity with non-specific interaction and better binding energy (−5.78 to −7.24 kcal/mol) and inhibition constant (57.87-4.90 μM). In conclusion, it can be interpreted that isolated polymethoxyflavones (Gardenin B, 5-Desmethylnobiletin, Gardenin E) could be taken up as a lead for target specific studies. Methanol extract and chloroform fraction prevails in all the tested activity therefore cumulative and composite intervention of polymethoxyflavones present in it reveals its pharmacological attributes and traditional value.

158

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomedicine & Pharmacotherapy - Volume 85, January 2017, Pages 444-456
نویسندگان
, , , ,