کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5588878 | 1569140 | 2017 | 17 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
PCR optimization and allele distribution of SNAC1 gene coding region in rice (Oryza sativa L.)
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کلمات کلیدی
QTLEtdBrNeutrality testquantitative traits lociPCoAdNTPTFsUPGMAPICOryza sativaABACTABSSRcetyltrimethylammonium bromide - اتلی تریمتیل آمونیوم برومیدethidium bromide - اتیدیوم برومایدAbiotic stress - استرس بی روحabscisic acid - اسید آبسزیکmarker assisted selection - انتخاب کمک کننده نشانگرMAS - بیشترPrincipal Coordinate Analysis - تجزیه و تحلیل مختصات اصلیGenetic diversity - تنوع ژنتیکیdeoxyribonucleoside triphosphate - دز اکسید ریبونوکلئوزید تری فسفاتSimple sequence repeats - دنباله ساده تکرار می شودunweighted pair group method with arithmetic mean - روش جفت گروه بدون وزن با میانگین ریاضیMicrosatellite - ریزماهوارهTranscription factors - عوامل رونویسیpolymorphic information content - محتوای اطلاعات پلی مورفUntranslated regions - مناطق غیر ترجمهUTR یا untranslated regions - منطقه ترجمه نشدهpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: PCR optimization and allele distribution of SNAC1 gene coding region in rice (Oryza sativa L.) PCR optimization and allele distribution of SNAC1 gene coding region in rice (Oryza sativa L.)](/preview/png/5588878.png)
چکیده انگلیسی
Drought is the most serious abiotic stress that limits crop production in rain-fed environments. In this study genetic diversity of SNAC1 gene investigated in a collection of 83 diverse rice accessions from different geographical origins. Amplification of SNAC1 gene exons was performed by combined hot start-touchdown PCR protocol. The average number of alleles was 9.5 alleles. A total of 6 rare alleles were identified from exons of SNAC1. The average gene diversity index, PIC (Polymorphism information content) and Shannon value were 0.8518, 0.8343 and 2.0469, respectively. Evolutionary study based on Ewens-Watterson test showed that exon 1 of SNAC1 gene was probability under genetic drift. To identify potential SSR markers in SNAC1 gene sequence, SSR distributions within rice SNAC1 gene sequence were mined. Totally 15 microsatellite loci were detected which tri-nucleotide motifs (8) was being most abundant, followed by di- (6) and tetra-nucleotide (1) motifs. Maximum loci were found in 3â² UTR (5) (Untranslated regions), followed by in 5â² UTR (4) and coding sequences (3 for each exon). The present study revealed genetic divergence of SNAC1 gene coding regions and also mined SSR distributions within SNAC1 gene sequence and introduced an optimized PCR method. This information can be used for the development of drought tolerant rice varieties.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Agri Gene - Volume 4, June 2017, Pages 30-36
Journal: Agri Gene - Volume 4, June 2017, Pages 30-36
نویسندگان
Narjes Tabkhkar, Babak Rabiei, Habibollah Samizadeh Lahiji, Maryam Hosseini Chaleshtori,