کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5590121 | 1570097 | 2016 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The Ciona intestinalis cleavage clock is independent of DNA methylation
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کلمات کلیدی
MBTwhole genome bisulfite sequencingWGBSCiona intestinalisTAO5hmCTETPBATDMR5mC - 5 سانتی متر5-Methylcytosine - 5-متیل سیتوزین5-hydroxymethylcytosine - 5-هیدروکسی متیل سیتوزینAChE - آهیAcetylcholinesterase - استیل کولین استرازMid-blastula transition - انتقال میانی بلواستولten-eleven translocation - ده یازده ترانزیتMass spectrometry - طیف سنجی جرمیDNA methylation - متیلاسیون DNADevelopmental stages - مراحل رشدDifferentially methylated region - منطقه متخلخل متفاوتی دارد
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The Ciona intestinalis cleavage clock is independent of DNA methylation The Ciona intestinalis cleavage clock is independent of DNA methylation](/preview/png/5590121.png)
چکیده انگلیسی
The initiation of embryonic gene expression in ascidian embryos appears to be tightly regulated by the number of DNA replication cycles. DNA methylation is thought to contribute to the clock mechanism that counts the rounds of DNA replication. We used mass spectrometry and whole genome bisulfite sequencing to characterize DNA methylation changes that occur in early developmental stages of the ascidian, Ciona intestinalis. We found that global DNA methylation in early Ciona development was static, and a base-wise comparison between the genomes of consecutive developmental stages found no DNA demethylation that was related to zygotic gene activation. Additionally, 5hmC was hardly detected by mass spectrometry in the developmental samples, suggesting a lack of demethylation mediated by ten eleven translocation (TET) methylcytosine dioxygenase in C. intestinalis. We conclude that DNA methylation is not involved in regulating DNA replication-dependent transcriptional activation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Genomics - Volume 108, Issues 3â4, October 2016, Pages 168-176
Journal: Genomics - Volume 108, Issues 3â4, October 2016, Pages 168-176
نویسندگان
Miho M. Suzuki, Tomoko Mori, Noriyuki Satoh,