|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5647131||1407077||2017||12 صفحه PDF||سفارش دهید||دانلود رایگان|
BackgroundRegulatory TÂ cells attenuate development of asthma in wild-type (WT) mice, with both naturally occurring regulatory T (nTreg) cells and inducible regulatory T (iTreg) cells exhibiting suppressive activity. When transferred into CD8-deficient (CD8â/â) recipients, both cell types enhanced development of allergen-induced airway hyperresponsiveness.ObjectiveWe sought to determine whether the pathways leading to enhancement of lung allergic responses by transferred nTreg and iTreg cells differed.MethodsnTreg cells (CD4+CD25+) were isolated from WT mice and iTreg cells were generated from WT CD4+CD25â TÂ cells after activation in the presence of TGF-Î² and transferred into sensitized CD8â/â recipients before challenge. Development of airway hyperresponsiveness, cytokine levels, and airway inflammation were monitored.ResultsTransfer of nTreg cells enhanced lung allergic responses, as did transfer of iTreg cells. Although anti-IL-13 reduced nTreg cell-mediated enhancement, it was ineffective in iTreg cell-mediated enhancement; conversely, anti-IL-17, but not anti-IL-13, attenuated the enhancement by iTreg cells. Recovered iTreg cells from the lungs of CD8â/â recipients were capable of IL-17 production and expressed high levels of signature genes of the TH17 pathway, RORÎ³t and Il17, whereas reduced expression of the Treg cell key transcription factor forkhead box p3 (Foxp3) was observed. InÂ vitro exogenous IL-6-induced IL-17 production in iTreg cells, and inÂ vivo conversion of transferred iTreg cells was dependent on recipient IL-6.ConclusionsiTreg cells, similar to nTreg cells, exhibit functional plasticity and can be converted from suppressor cellsÂ to pathogenic effector cells, enhancing lung allergic responses, but these effects were mediated through different pathways.
Journal: Journal of Allergy and Clinical Immunology - Volume 139, Issue 4, April 2017, Pages 1331-1342