کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5649371 | 1407123 | 2017 | 38 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Compartmentation of Mitochondrial and Oxidative Metabolism in Growing Hair Follicles: A Ring of Fire
ترجمه فارسی عنوان
تقسیم متابولیسم و اکسیداتیو در رشد فولیکول مو: یک حلقه آتش
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کلمات کلیدی
LTRTMRMTMRELysoTracker RedRh123ROS - ROSΔψ - Δρtetramethylrhodamine ethyl ester - اتیل استر استرهای تترامتیل رودامینRhodamine 123 - رودامین 123tetramethylrhodamine methyl ester - متیل استر تترامتیل رودامینMitochondrial membrane potential - پتانسیل غشای میتوکندریPropidium iodide - پروتئین یدیدReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
امراض پوستی
چکیده انگلیسی
Little is known about the energetics of growing hair follicles, particularly in the mitochondrially abundant bulb. Here, mitochondrial and oxidative metabolism was visualized by multiphoton and light sheet microscopy in cultured bovine hair follicles and plucked human hairs. Mitochondrial membrane potential (ÎΨ), cell viability, reactive oxygen species (ROS), and secretory granules were assessed with parameter-indicating fluorophores. In growing follicles, lower bulb epithelial cells had high viability, and mitochondria were polarized. Most epithelially generated ROS co-localized with polarized mitochondria. As the imaging plane captured more central and distal cells, ÎΨ disappeared abruptly at a transition to a nonfluorescent core continuous with the hair shaft. Approaching the transition, ÎΨ and ROS increased, and secretory granules disappeared. ROS and ÎΨ were strongest in a circumferential paraxial ring at putative sites for formation of the outer cortex/cuticle of the hair shaft. By contrast, polarized mitochondria in dermal papillar fibroblasts produced minimal ROS. Plucked hairs showed a similar abrupt transition of degranulation/depolarization near sites of keratin deposition, as well as an ROS-generating paraxial ring of fire. Hair movement out of the follicle appeared to occur independently of follicular bulb bioenergetics by a tractor mechanism involving the inner and outer root sheaths.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Investigative Dermatology - Volume 137, Issue 7, July 2017, Pages 1434-1444
Journal: Journal of Investigative Dermatology - Volume 137, Issue 7, July 2017, Pages 1434-1444
نویسندگان
John J. Lemasters, Venkat K. Ramshesh, Gregory L. Lovelace, John Lim, Graham D. Wright, Duane Harland, Thomas L. Jr.,