کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5649749 | 1407130 | 2017 | 37 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enhanced Neurogenic Biomarker Expression and Reinnervation in Human Acute Skin Wounds Treated by Electrical Stimulation
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
NGFLAMP2PGP9.5CK20BDNF - BDNF یا فاکتور نورونزایی مشتقشده از مغز Small interfering RNA - RNA تداخل کوچکsiRNA - siRNAIHC - ایمونوهیستوشیمیImmunohistochemistry - ایمونوهیستوشیمیElectrical stimulation - تحریک الکتریکیRetinoic acid - رتینوئیک اسیدcytokeratin 20 - سیتوکراتین 20nerve growth factor - فاکتور رشد عصبBrain-derived neurotrophic factor - فاکتور نوروتروفی مشتق شده از مغزlysosomal-associated membrane protein 2 - پروتئین غشای مرتبط با لیزوزوم 2protein gene product 9.5 - ژن پروتئین محصول 9.5Control - کنترل
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
امراض پوستی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Electrical stimulation (ES) is known to promote cutaneous healing; however, its ability to regulate reinnervation remains unclear. First, we show that ES treatment of human acute cutaneous wounds (n = 40) increased reinnervation. Next, to define neurophysiologic mechanisms through which ES affects repair, microarray analysis of wound biopsy samples was performed on days 3, 7, 10, and 14 after wounding. This identified neural differentiation biomarkers TUBB3 (melanocyte development and neuronal marker) and its upstream molecule FIG4 (phosphatidylinositol (3,5)-bisphosphate 5-phosphatase) as significantly up-regulated after ES treatment. To demonstrate a functional ES-TUBB3 axis in cutaneous healing, we showed increased TUBB3+ melanocytes and melanogenesis plus FIG4 and nerve growth factor expression, suggesting higher cellular differentiation. In support of this role of ES to regulate neural crest-derived cell fate and differentiation in vivo, knockdown of FIG4 in neuroblastoma cells resulted in vacuologenesis and cell degeneration, whereas ES treatment after FIG4-small interfering RNA transfection enhanced neural differentiation, survival, and integrity. Further characterization showed increased TUBB3+ and protein gene product 9.5+ Merkel cells during in vivo repair, after ES. We demonstrate that ES contributes to increased expression of neural differentiation biomarkers, reinnervation, and expansion of melanocyte and Merkel cell pool during repair. Targeted ES-assisted acceleration of healing has significant clinical implications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Investigative Dermatology - Volume 137, Issue 3, March 2017, Pages 737-747
Journal: Journal of Investigative Dermatology - Volume 137, Issue 3, March 2017, Pages 737-747
نویسندگان
Anil Sebastian, Susan W. Volk, Poonam Halai, James Colthurst, Ralf Paus, Ardeshir Bayat,