کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5822377 | 1117940 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Repeated exposure to 5D9, an inhibitor of 3D polymerase, effectively limits the replication of foot-and-mouth disease virus in host cells
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کلمات کلیدی
BMEphosphate buffered saline with tween-203Dpol3D polymeraseBHKFMDVRdRpFMDRIPAATCCRT-PCRNTPPBSThpi3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-difenyltetrazolium bromideDMSO - DMSOMOI - MEMTT - MTTRNA dependent RNA polymerase - RNA پلیمراز وابسته به RNAribonucleic acid - اسید ریبونوکلئیکRNA - اسید ریبونوکلئیکQSPR - برقراری ارتباط کمی بین ساختار و خواص مولکولbaby hamster kidney - بچه هامستر کلیهFoot-and-mouth disease - تب برفکی، بیماری دهان و پاdimethylsulphoxide - دی متیل سولفوکسیدQuantitative structure–property relationship - رابطه ساختاری و مالکیت کمیhours post infection - ساعت پس از عفونتradioimmunoprecipitation assay - سنجش radioimmunoprecipitationAntiviral - ضد ویروسیAmerican Type Culture Collection - مجموعه فرهنگی نوع آمریکاییnucleoside triphosphate - نوکلئوزید تری فسفاتReverse transcriptase-polymerase chain reaction - واکنش زنجیره ای واکنش زنجیره ای واکنش زنجیره ایFoot-and-mouth disease virus - ویروس آنفلوآنزای مرغیFoot-and-mouth disease virus (FMDV) - ویروس آنفلوآنزای مرغی (FMDV)multiplicity of infection - چندین عفونت
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Foot-and-mouth disease (FMD) is a highly contagious disease of livestock caused by a highly variable RNA virus (FMDV) that has seven serotypes and more than sixty subtypes. Both prophylactic and post-infection means of controlling the disease outbreak, including universally applicable vaccines and emergency response measures such as therapeutic treatments, are on high demand. In this study, we analyzed the long-term exposure outcome to a previously identified inhibitor of 3D polymerase (FMDV 3Dpol) for controlling FMDV infection and for the selection of resistance mutants. The results showed that no escape mutant viruses were isolated from FMDV A24 Cruzeiro infections in cell culture treated with gradually increasing concentrations of the antiviral compound 5D9 (4-chloro-Nâ²-thieno, [2,3-d]pyrimidin-4-ylbenzenesulfonohydrazide) over ten passages. Biochemical and plaque assays revealed that when 5D9 was used at concentrations within a non-toxic range in cells, it drove the virus to undetectable levels at passage eight to ten. This is in contrast with observations made on parallel control (untreated) passages exhibiting fully viable and stable virus progenies. Collectively, the results demonstrated that under the experimental conditions, treatment with 5D9 does not confer a resistant phenotype and the virus is unable to evade the antiviral effect of the inhibitor. Further efforts using quantitative structure-property relationship (QSPR) based modifications of the 5D9 compound may result in the successful development of an effective in vivo antiviral drug targeting FMDV.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Antiviral Research - Volume 98, Issue 3, June 2013, Pages 380-385
Journal: Antiviral Research - Volume 98, Issue 3, June 2013, Pages 380-385
نویسندگان
Devendra K. Rai, Elizabeth A. Schafer, Kamalendra Singh, Mark A. McIntosh, Stefan G. Sarafianos, Elizabeth Rieder,