کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5832010 | 1559754 | 2016 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Anti-TNF-α and anti-arthritic effect of patuletin: A rare flavonoid from Tagetes patula
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کلمات کلیدی
NF-κBPatuletinTNFImmunosuppressivePMNsAP-1iNOSERKTCrATFNFATJnkCOXMMPAIAc-Jun N-terminal kinases - C-Jun N-terminal kinasesMAPK - MAPKROS - ROSAdjuvant induced arthritis - آرتروز ناشی از ادجوانتRheumatoid arthritis - آرتریتروماتوئیدcyclooxygenase - آنزیم سیکلواکسیژنازSprague Dawley - اسپراگ داولیinterleukin - اینترلوکینPolymorphonuclear cells - سلولهای پلیمورفونیکinducible nitric oxide synthase - سنتاز اکسید نیتریک القاییextracellular signal regulated kinases - سیگنال خارج سلولی kinases را تنظیم می کندrheumatoid factor - عامل روماتوئیدnuclear factor of activated T-cells - عامل هسته سلول های فعال شده فعال استtumor necrosis factor - فاکتور نکروز تومورnuclear factor kappa B - فاکتور هسته ای کاپا Bactivating transcription factor - فعال کردن عامل رونویسیmatrix metalloproteinase - ماتریکس متالوپروتئینازNitric oxide - نیتریک اکسیدPeroxide - پراکسیدactivator protein 1 - پروتئین فعال کننده 1Reactive oxygen species - گونههای فعال اکسیژنT-cell receptor - گیرنده لنفوسیت T
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Rheumatoid arthritis (RA) poses a serious health problem as a chronic autoimmune joint disease with significant mortality and morbidity. Proinflammatory cytokines TNF-α and IL-1β, reactive oxygen species (ROS), and activated CD4+ T-cells play key roles in the progression of arthritis. The aim of the study is to evaluate the in vitro and in vivo immunomodulatory and anti-arthritic effect of flavonoid patuletin, isolated from Tagetes patula. ELISA was applied for quantification of TNF-α and IL-1β. Intracellular and extracellular ROS production from phagocytes was measured by the chemiluminescence technique. Proliferation of T-cells was observed using a liquid scintillation counter. Cytotoxicity was assessed by a MTT assay. The serological and histological analysis studies were performed using a rodent model of adjuvant-induced arthritis (AIA). Expression of p38 and NF-κB after treatment of compound was observed by western blotting. Patuletin showed potent inhibitory effects on TNF-α in vitro as well as inhibited the production of both cytokines in vivo. It also showed potent suppression of proliferation of T-cells and significantly inhibited the extracellular and intracellular ROS production. Patuletin revealed significant anti-inflammatory and anti-arthritic activities in the rodent model of adjuvant-induced arthritis (AIA). Histologically, it causes mild bone destruction compared to the arthritic control group, thus representing its anti-arthritic potential. Based on these studies, patuletin could be considered as a potential immunosuppressive and anti-arthritic lead candidate.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Immunopharmacology - Volume 36, July 2016, Pages 232-240
Journal: International Immunopharmacology - Volume 36, July 2016, Pages 232-240
نویسندگان
Almas Jabeen, M. Ahmed Mesaik, Shabana U. Simjee, Lubna Lubna, Samina Bano, Shaheen Faizi,