کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5847943 | 1561613 | 2015 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Functional and mechanistic investigation of Shikonin in scarring
ترجمه فارسی عنوان
بررسی کارکردی و مکانیکی شیکونین در زخم
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کلمات کلیدی
αSMAFCShsfDAPIqRT-PCRSfMBcl-2NaOHCOL1A1DMEMCol3a1terminal deoxynucleotidyl transferase dUTP nick end labelling4′,6-diamidino-2-phenylindole - 4 '، 6-دیامیدینو-2-فنیلینولDMSO - DMSOERK1/2 - ERK1 / 2JNK1/2 - JNK1 / 2p-ERK1/2 - P-ERK1 / 2SDS–PAGE - SDS-PAGEAlpha-smooth muscle actin - آکتا عضله آلفا صافsodium dodecyl sulphate polyacrylamide gel electrophoresis - الکتروفورز ژل پلی اکریللید سدیم دودسیل سولفاتShikonin - تماشایTUNEL - تونلApoptosis - خزان یاختهایstandard error of the mean - خطای استاندارد میانگینDimethyl sulfoxide - دیمتیل سولفواکسیدserum free medium - سرم رایگان استfetal calf serum - سرم گوساله جنینB-cell lymphoma 2 - لنفوم سلول B 2Dulbecco’s modified eagle’s medium - محیط عقاب اصلاح شده DulbeccoSEM - مدل معادلات ساختاری / میکروسکوپ الکترونی روبشیsodium hydroxide - هیدروکسید سدیمQuantitative reverse transcriptase polymerase chain reaction - واکنش زنجیره ای پلی مراز ترانس کریتاز معکوس کمیCaspases - کاسپازhuman skin keratinocytes - کراتینوسیت پوست انسانextracellular signal-regulated kinase 1 and 2 - کیناز 1 و 2 تنظیم شده با سیگنال خارج سلولی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
چکیده انگلیسی
Scarring is a significant medical burden; financially to the health care system and physically and psychologically for patients. Importantly, there have been numerous case reports describing the occurrence of cancer in burn scars. Currently available therapies are not satisfactory due to their undesirable side-effects, complex delivery routes, requirements for long-term use and/or expense. Radix Arnebiae (Zi Cao), a perennial herb, has been clinically applied to treat burns and manage scars for thousands of years in Asia. Shikonin, an active component extracted from Radix Arnebiae, has been demonstrated to induce apoptosis in cancer cells. Apoptosis is an essential process during scar tissue remodelling. It was therefore hypothesized that Shikonin may induce apoptosis in scar-associated cells. This investigation presents the first detailed in vitro study examining the functional responses of scar-associated cells to Shikonin, and investigates the mechanisms underlying these responses. The data obtained suggests that Shikonin inhibits cell viability and proliferation and reduces detectable collagen in scar-derived fibroblasts. Further investigation revealed that Shikonin induces apoptosis in scar fibroblasts by differentially regulating the expression of caspase 3, Bcl-2, phospho-Erk1/2 and phospho-p38. In addition, Shikonin down-regulates the expression of collagen I, collagen III and alpha-smooth muscle actin genes hence attenuating collagen synthesis in scar-derived fibroblasts. In summary, it is demonstrated that Shikonin induces apoptosis and decreases collagen production in scar-associated fibroblasts and may therefore hold potential as a novel scar remediation therapy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemico-Biological Interactions - Volume 228, 25 February 2015, Pages 18-27
Journal: Chemico-Biological Interactions - Volume 228, 25 February 2015, Pages 18-27
نویسندگان
Yan Xie, Chen Fan, Ying Dong, Emily Lynam, David I. Leavesley, Kun Li, Yonghua Su, Yinxue Yang, Zee Upton,