کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5855707 | 1562122 | 2016 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Corn rootworm-active RNA DvSnf7: Repeat dose oral toxicology assessment in support of human and mammalian safety
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کلمات کلیدی
dsRNAGM cropRNA interference - RNA تداخل کنندهSmall interfering RNA - RNA تداخل کوچکdouble stranded RNA - RNA دو رشته ایRNAi - RNA سرکوبگر،RNA مداخلهگر، RNA خاموش کنندهmicro RNA - RNA میکروsiRNA - siRNARNA - اسید ریبونوکلئیکribonucleic acid - اسید ریبونوکلئیکGenetically modified - اصلاح ژنتیکیbase pair - جفت پایهGene Suppression - سرکوب ژنGenetically modified crop - محصول اصلاح شده ژنتیکیMiRNA - میکروRNA، ریزآرانای، miRNA
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Genetically modified (GM) crops have been developed and commercialized that utilize double stranded RNAs (dsRNA) to suppress a target gene(s), producing virus resistance, nutritional and quality traits. MON 87411 is a GM maize variety that leverages dsRNAs to selectively control corn rootworm through production of a 240 base pair (bp) dsRNA fragment targeting for suppression the western corn rootworm (Diabrotica virgifera virgifera) Snf7 gene (DvSnf7). A bioinformatics assessment found that endogenous corn small RNAs matched â¼450 to 2300 unique RNA transcripts that likely code for proteins in rat, mouse, and human, demonstrating safe dsRNA consumption by mammals. Mice were administered DvSnf7 RNA (968 nucleotides, including the 240 bp DvSnf7 dsRNA) at 1, 10, or 100Â mg/kg by oral gavage in a 28-day repeat dose toxicity study. No treatment-related effects were observed in body weights, food consumption, clinical observations, clinical chemistry, hematology, gross pathology, or histopathology endpoints. Therefore, the No Observed Adverse Effect Level (NOAEL) for DvSnf7 RNA was 100Â mg/kg, the highest dose tested. These results demonstrate that dsRNA for insect control does not produce adverse health effects in mammals at oral doses millions to billions of times higher than anticipated human exposures and therefore poses negligible risk to mammals.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Regulatory Toxicology and Pharmacology - Volume 81, November 2016, Pages 57-68
Journal: Regulatory Toxicology and Pharmacology - Volume 81, November 2016, Pages 57-68
نویسندگان
Jay S. Petrick, Gregory E. Frierdich, Stephanie M. Carleton, Colton R. Kessenich, Andre Silvanovich, Yuanji Zhang, Michael S. Koch,