کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5861999 | 1133771 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Way forward in case of a false positive in vitro genotoxicity result for a cosmetic substance?
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
CNDnIFSPBMPHGTXNNKTOLPiperonyl butoxideCyclo12-O-tetradecanoylphorbol-13-acetateTRIHCABAPKNNSDF2NFtPAWy-14643phenobarbital sodium2-nitrofluorene - 2-نیتروفلوئورن4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone - 4- (methylnitrosamino) -1- (3-pyridyl) -1-butanoneAFB1 - AFB 1d-mannitol - D-منیتولPCA - PCAMTT test - آزمون MTTAflatoxin B1 - آفلاتوکسین B1chromosome aberration - انحراف کروموزومBenzo(a)pyrene - بنزو (a) پیرنهPrinciple component analysis - تجزیه و تحلیل اجزای اصلhierarchical clustering analysis - تجزیه و تحلیل خوشه ای سلسله مراتبیTriclosan - تری کلوزانTolbutamide - تولوباتیدdiclofenac sodium - دیکلوفناک سدیمCyclophosphamide - سیکلوفسفامید Non-genotoxic - غیر ژنوتوکسیکLOO - لئوMan - مردCosmetic ingredients - مواد آرایشیMicronucleus - میکروسکوپk-nearest neighbours - نزدیک ترین همسایگانNifedipine - نیفدیپینGenotoxic - ژنوتوکسیکclonidine - کلونیدین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The currently used regulatory in vitro mutagenicity/genotoxicity test battery has a high sensitivity for detecting genotoxicants, but it suffers from a large number of irrelevant positive results (i.e. low specificity) thereby imposing the need for additional follow-up by in vitro and/or in vivo genotoxicity tests. This could have a major impact on the cosmetic industry in Europe, seen the imposed animal testing and marketing bans on cosmetics and their ingredients. Afflicted, but safe substances could therefore be lost. Using the example of triclosan, a cosmetic preservative, we describe here the potential applicability of a human toxicogenomics-based in vitro assay as a potential mechanistically based follow-up test for positive in vitro genotoxicity results. Triclosan shows a positive in vitro chromosomal aberration test, but is negative during in vivo follow-up tests. Toxicogenomics analysis unequivocally shows that triclosan is identified as a compound acting through non-DNA reactive mechanisms. This proof-of-principle study illustrates the potential of genome-wide transcriptomics data in combination with in vitro experimentation as a possible weight-of-evidence follow-up approach for de-risking a positive outcome in a standard mutagenicity/genotoxicity battery. As such a substantial number of cosmetic compounds wrongly identified as genotoxicants could be saved for the future.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 28, Issue 1, February 2014, Pages 54-59
Journal: Toxicology in Vitro - Volume 28, Issue 1, February 2014, Pages 54-59
نویسندگان
Tatyana Y. Doktorova, Gamze Ates, Mathieu Vinken, Tamara Vanhaecke, Vera Rogiers,