کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5863164 | 1133806 | 2011 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The intra- and inter-laboratory reproducibility and predictivity of the KeratinoSens assay to predict skin sensitizers in vitro: Results of a ring-study in five laboratories
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کلمات کلیدی
BLRWLRECVAMMUSSTDNCBh-CLATLLNART-PCRICCVAMSOPNrf2IC50SLSwithin laboratory reproducibility2,4-dinitrochlorobenzene - 2،4-dinitrochlorobenzenekeap1 - buy1DMSO - DMSOhuman cell line activation test - آزمایش فعال سازی سلول انسانی انسانLocal lymph node assay - آزمایش گره لنفاوی محلیregistration, evaluation, authorization and restriction of chemicals - ثبت نام، ارزیابی، مجوز و محدودیت مواد شیمیاییSkin sensitization - حساسیت پوستDimethylsulfoxide - دیمتیل سولفواکسیدREACH - رسیدنstandard operating procedure - روش های عملیاتی استانداردsodium lauryl sulphate - سدیم لوریل سولفاتPredictive capacity - ظرفیت پیش بینی شدهNuclear factor-erythroid 2-related factor 2 - عامل فاکتور هسته ای - عامل 2 وابسته به erythroid 2antioxidant response element - عنصر پاسخ آنتی اکسیدانTransferability - قابل انتقالARE - هستندMCI - همراه اولreverse transcriptase polymerase chain reaction - واکنش زنجیره ای پلی مراز ترانس کریتاز معکوسKelch-like ECH-associated protein 1 - پروتئین مرتبط با ECH کلچ 1Reporter gene - ژن خبرنگارInteragency Coordinating Committee on the Validation of Alternative Methods - کمیته هماهنگی بین ادارات اعتبار سنجی روش های جایگزین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Due to regulatory constraints and ethical considerations, research on alternatives to animal testing to predict the skin sensitization potential of novel chemicals has gained a high priority. Accordingly, different in vitro, in silico and in chemico approaches have been described in the scientific literature to achieve this goal. To replace regulatory approved animal tests, these alternatives need to be transferable to other labs, their within and between laboratory reproducibility must be assured, and their predictivity should be high. The KeratinoSens assay is a cell-based reporter gene assay to screen substances with a full dose-response assessment. It is based on a stable transgenic keratinocyte cell line. The induction of a luciferase gene under the control of the antioxidant response element (ARE) derived from the human AKR1C2 gene is determined. Here we report on the results of a ring-study with five laboratories performing the KeratinoSens assay on a set of 28 test substances. The assay was found to be easily transferable to all laboratories. Overall both the qualitative (sensitizer/non-sensitizer categorization) and the quantitative (concentration for significant gene induction) results were reproducible between laboratories. A detailed analysis of the transferability, the within- and between laboratory reproducibility and the predictivity is presented.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 25, Issue 3, April 2011, Pages 733-744
Journal: Toxicology in Vitro - Volume 25, Issue 3, April 2011, Pages 733-744
نویسندگان
Andreas Natsch, Caroline Bauch, Leslie Foertsch, Frank Gerberick, Kimberly Norman, Allison Hilberer, Heather Inglis, Robert Landsiedel, Stefan Onken, Hendrik Reuter, Andreas Schepky, Roger Emter,