کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5904365 | 1157979 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Phytic acid and myo-inositol support adipocyte differentiation and improve insulin sensitivity in 3T3-L1 cells
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کلمات کلیدی
PPAR2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-d-glucoseSREBP2-NBDGGLUTPKBIRSFASFBSPBSPI3KDMEMC/EBP - C / EBPDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoAdipogenesis - آدیپوژنزfatty acid synthase - اسید چرب سنتازinsulin receptor substrate - انسولین بستر گیرندهanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of variancetriacylglycerol - تری آسیل گلیسرول Glucose uptake - جذب گلوکزInsulin sensitivity - حساسیت به انسولینGlucose transporter - حمل و نقل گلوکزBCs - روند BCsfetal bovine serum - سرم جنین گاوbovine calf serum - سرم گوساله گوساله3T3-L1 cells - سلول های 3T3-L1phosphoinositide 3-kinase - فسفینوزیتید 3-کینازPhytic acid - فیتیک اسید، هگزوفسفات اینوزیتولPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریMyo-inositol - میو-آنزیتولpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازCCAAT/enhancer-binding protein - پروتئین CCAAT / تقویت کنندهSterol regulatory element-binding protein - پروتئین اتصال دهنده پروتئین Sterol Regulatoryprotein kinase B - پروتئین کیناز Bperoxisome proliferator-activated receptor - گیرنده فعال فعال پروکسیوم
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
علوم غدد
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Phytic acid, also known as myo-inositol hexaphosphate, has been shown to lower blood glucose levels and to improve insulin sensitivity in rodents. We investigated the effects of phytic acid and myo-inositol on differentiation, insulin-stimulated glucose uptake, and lipolysis of adipocytes to test the hypothesis that the antidiabetic properties of phytic acid and myo-inositol are mediated directly through adipocytes. 3T3-L1 cells were treated with 10, 50, or 200 μmol/L of phytic acid or myo-inositol. Oil Red O staining and an intracellular triacylglycerol assay were used to determine lipid accumulation during adipocyte differentiation. Immunoblotting and real-time polymerase chain reaction (PCR) were performed to evaluate expression of transcription factors, a target protein, and insulin signaling molecules. Phytic acid and myo-inositol exposures increased lipid accumulation in a dose-dependent manner (P < .01). The expression of key transcription factors associated with adipocyte differentiation, such as peroxisome proliferator-activated receptor γ (PPARγ) and sterol regulatory element-binding protein 1c, and the expression of fatty acid synthase increased upon treatments with phytic acid and myo-inositol (P < .05). Insulin-stimulated glucose uptake in mature adipocytes increased with phytic acid and myo-inositol treatments (P < .01). In addition, mRNA levels of insulin receptor substrate 1 (IRS1), mRNA levels of glucose transporter 4, and phosphorylation of tyrosine in IRS1 increased upon phytic acid and myo-inositol treatments. In fully differentiated adipocytes, phytic acid and myo-inositol reduced basal lipolysis dose dependently (P < .01). These results suggest that phytic acid and myo-inositol increase insulin sensitivity in adipocytes by increasing lipid storage capacity, improving glucose uptake, and inhibiting lipolysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Nutrition Research - Volume 34, Issue 8, August 2014, Pages 723-731
Journal: Nutrition Research - Volume 34, Issue 8, August 2014, Pages 723-731
نویسندگان
Jin Nam Kim, Sung Nim Han, Hye- Kyeong Kim,