کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6051074 1583306 2014 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The role of GPC5 in lung metastasis of salivary adenoid cystic carcinoma
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی دندانپزشکی، جراحی دهان و پزشکی
پیش نمایش صفحه اول مقاله
The role of GPC5 in lung metastasis of salivary adenoid cystic carcinoma
چکیده انگلیسی


- Most of HSPGs expressions were decreased in SACC-M cells, only GPC5 increased more than 3-fold in SACC-M compared with SACC-2 and SACC-83 cells.
- Immunohistochemical staining showed higher expression of GPC5 in SACC with lung metastasis compared with SACC without lung metastasis.
- The lung metastasis of SACC-M cells in nude mice was obviously decreased after GPC5 silencing, and GPC5 silencing was independent of SACC-M cell proliferation.

ObjectiveProteoglycans play a crucial role in salivary adenoid cystic carcinoma (SACC) tumorigenesis, neurotropic growth and lung metastasis. In this study, we investigated the role of GPC5 in the lung metastasis of SACC.DesignThe expression of heparan sulfate proteoglycans (HSPGs) was detected in SACC-M (high lung-metastatic cell line), SACC-2 (low lung-metastatic cell line) and SACC-83 (low lung-metastatic cell line) cells by relative quantitative Real-Time PCR. The expression of GPC5 was detected by immunofluorescence and Western blot analysis in SACC-M, SACC-2 and SACC-83 cells, and by immunohistochemical analysis in primary tumours from 16 cases of SACC patients with or without lung metastasis. GPC5 expression was silenced in SACC-M cells, cell proliferation and tumour growth was evaluated by MTT assay and nude mice model, respectively.ResultsExpression of most HSPGs was decreased in SACC-M cells, but GPC5 expression increased 3.24-fold and 815.69-fold (more than 3-fold) in SACC-M compared with SACC-2 and SACC-83 cells, respectively. Immunohistochemical analysis showed higher expression of GPC5 in SACC with lung metastasis compared to SACC without lung metastasis. The lung metastasis of SACC-M cells in nude mice was obviously decreased after GPC5 silencing (P < 0.05), although GPC5 silencing had no significant effect on SACC-M cell proliferation.ConclusionGPC5 may contribute to lung metastasis of SACC.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Oral Biology - Volume 59, Issue 11, November 2014, Pages 1172-1182
نویسندگان
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