کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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6055250 | 1198796 | 2013 | 7 صفحه PDF | دانلود رایگان |
SummaryObjectivesMicroRNAs (miRNAs) are endogenous small non-coding RNAs that negatively regular target gene expression by RNA interference. The processing of the pre-miRNA hairpin generates a miRNA duplex, which consists of a miRNA (guide strand) and a miRNAâ (passenger strand). miR-31 is an oncogenic miRNA and is up-regulated in oral squamous cell carcinoma (OSCC). miR-31â shows a high level of conservation across species and, based on this, this study hypothesized that miR-31â is a functional miRNA.Materials and MethodsThe expression of miR-31 and miR-31â in OSCC tissues and oral cells were analyzed. Functional studies were performed on OSCC cells.ResultsmiR-31â is up-regulated in OSCC tissues, but its expression is less abundant than miR-31. miR-31â decreases the proliferation and migration of both SAS and Fadu cells. Furthermore, miR-31â targets the 3â²UTR of RhoA and is able to down-regulate RhoA expression. Knockdown of RhoA expression is known to decrease the proliferation and migration of OSCC cells. However, up-regulation of both miR-31 and miR-31â by delivery of pre-mir-31 does still enhance OSCC oncogenicity.ConclusionmiR-31â is a functional miRNA involving in regulating RhoA, and the activity of miR-31â's activity seems to counteract the functions of miR-31 during OSCC tumorigenesis.
Journal: Oral Oncology - Volume 49, Issue 1, January 2013, Pages 27-33