|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|6062306||1201839||2016||18 صفحه PDF||سفارش دهید||دانلود رایگان|
BackgroundSubcutaneous allergen-specific immunotherapy is a standard route for the immunotherapy of allergic diseases. It modulates the course of allergy and can generate long-term remission. However, subcutaneous allergen-specific immunotherapy can also induce anaphylaxis in some patients, and therefore additional routes of administration should be investigated to improve the safety and tolerability of immunotherapy.ObjectiveWe sought to determine whether epicutaneous treatment with antigen in the presence of a Toll-like receptor 9 agonist can suppress TH2-mediated responses in an antigen-specific manner.MethodsEpicutaneous immunization was performed by applying a skin patch soaked with ovalbumin (OVA) plus CpG, and its suppressor activity was determined by using the mouse model of atopic dermatitis. Finally, adoptive cell transfers were implemented to characterize the regulatory cells that are induced by epicutaneous immunization.ResultsEpicutaneous immunization with OVA and CpG reduces the production of OVA-specific IgE and increases the synthesis of OVA-specific IgG2a antibodies in an antigen-specific manner. Moreover, eosinophil peroxidase activity in the skin and production of IL-4, IL-5, IL-10, and IL-13 are suppressed. The observed reduction of IgE synthesis is transferable with T-cell receptor (TCR) Î±Î²+CD4+CD25â cells, whereas IgG2a production is dependent on both TCRÎ±Î²+ and TCRÎ³Î´+ TÂ cells. Further experiments show that the described phenomenon is myeloid differentiation primary response 88, IFN-Î³, and IL-17A dependent. Finally, the results suggest that epicutaneous immunization with OVA and CpG decreases the synthesis of OVA-specific IgE and skin eosinophil peroxidase activity in mice with ongoing skin allergy.ConclusionEpicutaneous application of protein antigen in theÂ presence of adjuvant could be an attractive needle-free and self-administered immunotherapy for allergic diseases.
Journal: Journal of Allergy and Clinical Immunology - Volume 138, Issue 1, July 2016, Pages 262-273.e6