کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6074502 | 1203487 | 2016 | 38 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of Autoantigen Epitopes in Alopecia Areata
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کلمات کلیدی
PBMCCTLSFCICSELISPOTalopecia areatagp100TrichohyalinEnzyme-linked immunospot - ایمنی مصنوعی مرتبط با آنزیمTyr - تیرTyrosinase - تیروزیناز conditioned media - رسانه های متعلق بهSpot-forming cell - سلول تشکیل دهنده نقطهPeripheral blood mononuclear cell - سلول تک هسته ای خون محیطیHair follicle - فولیکول موCytotoxic T lymphocytes - لنفوسیت های T سیتوتوکسیک
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
امراض پوستی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Alopecia areata (AA) is believed to be a cell-mediated autoimmune hair loss disease. Both CD4 and cytotoxic CD8 T cells (CTLs) are important for the onset and progression of AA. Hair follicle (HF) keratinocyte and/or melanocyte antigen epitopes are suspected potential targets of autoreactive CTLs, but the specific epitopes have not yet been identified. We investigated the potential for a panel of known epitopes, expressed by HF keratinocytes and melanocytes, to induce activation of CTL populations in peripheral blood mononuclear cells. Specific synthetic epitopes derived from HF antigens trichohyalin and tyrosinase-related protein-2 induced significantly higher frequencies of response in AA CTLs compared with healthy controls (IFN-gamma secretion). Apoptosis assays revealed conditioned media from AA peripheral blood mononuclear cells stimulated with trichohyalin peptides elevated the expression of apoptosis markers in primary HF keratinocytes. A cytokine array revealed higher expression of IL-13 and chemokine ligand 5 (CCL5, RANTES) from AA peripheral blood mononuclear cells stimulated with trichohyalin peptides compared with controls. The data indicate that AA affected subjects present with an increased frequency of CTLs responsive to epitopes originating from keratinocytes and melanocytes; the activated CTLs secreted soluble factors that induced apoptosis in HF keratinocytes. Potentially, CTL response to self-antigen epitopes, particularly trichohyalin epitopes, could be a prognostic marker for human AA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Investigative Dermatology - Volume 136, Issue 8, August 2016, Pages 1617-1626
Journal: Journal of Investigative Dermatology - Volume 136, Issue 8, August 2016, Pages 1617-1626
نویسندگان
Eddy H.C. Wang, Mei Yu, Trisia Breitkopf, Noushin Akhoundsadegh, Xiaojie Wang, Feng-Tao Shi, Gigi Leung, Jan P. Dutz, Jerry Shapiro, Kevin J. McElwee,