کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6074511 | 1203487 | 2016 | 30 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Pelota Regulates Epidermal Differentiation by Modulating BMP and PI3K/AKT Signaling Pathways
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کلمات کلیدی
PelotaPI3KpSmadEPBPhospho-SmadFLGpAktAkt - آکتTAM - تامtamoxifen - تاموکسیفنembryonic day - روز جنینیphospho-Akt - فسفات آکتphosphoinositide 3-kinase - فسفینوزیتید 3-کینازfilaggrin - فیلهگرینStratum corneum - لایه شاخی، استراتوم کورنئومepidermal permeability barrier - مانع نفوذ اپیدرمیBMP - مدیریت فرایند کسب و کارPelo - موcornified envelope - پاکت زده شدهBone morphogenetic protein - پروتئین مورفوژنیک استخوانprotein kinase B - پروتئین کیناز B
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
امراض پوستی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The depletion of evolutionarily conserved pelota protein causes impaired differentiation of embryonic and spermatogonial stem cells. In this study, we show that temporal deletion of pelota protein before epidermal barrier acquisition leads to neonatal lethality due to perturbations in permeability barrier formation. Further analysis indicated that this phenotype is a result of failed processing of profilaggrin into filaggrin monomers, which promotes the formation of a protective epidermal layer. Molecular analyses showed that pelota protein negatively regulates the activities of bone morphogenetic protein and phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) signaling pathways in the epidermis. To address whether elevated activities of bone morphogenetic protein and PI3K/AKT signaling pathways were the cause for the perturbed epidermal barrier in Pelo-deficient mice, we made use of organotypic cultures of skin explants from control and mutant embryos at embryonic day 15.5. Inhibition of PI3K/AKT signaling did not significantly affect the bone morphogenetic protein activity. However, inhibition of bone morphogenetic protein signaling caused a significant attenuation of PI3K/AKT activity in mutant skin and, more interestingly, the restoration of profilaggrin processing and normal epidermal barrier function. Therefore, increased activity of the PI3K/AKT signaling pathway in Pelo-deficient skin might conflict with the dephosphorylation of profilaggrin and thereby affect its proper processing into filaggrin monomers and ultimately the epidermal differentiation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Investigative Dermatology - Volume 136, Issue 8, August 2016, Pages 1664-1671
Journal: Journal of Investigative Dermatology - Volume 136, Issue 8, August 2016, Pages 1664-1671
نویسندگان
Manar Elkenani, Gunsmaa Nyamsuren, Priyadharsini Raju, Kifayathullah Liakath-Ali, Aicha Hamdaoui, Aleksandra Kata, Ralf Dressel, Thomas Klonisch, Fiona M. Watt, Wolfgang Engel, James A. Thliveris, D.V. Krishna Pantakani, Ibrahim M. Adham,