کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6116964 | 1591550 | 2016 | 51 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Glycolytic pathway affects differentiation of human monocytes to regulatory macrophages
ترجمه فارسی عنوان
مسیر گلیکولیتی بر تمایز تک سلولی انسان به ماکروفاژهای نظارتی اثر می گذارد
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کلمات کلیدی
DCALPSIDO1TCAVEGFA2-deoxy-d-glucoseIFN-γPDKMMP9CSF1M-CSF2-DG - 2 DGinterferon-gamma - اینترفرون گاماIndoleamine 2,3-dioxygenase 1 - ایندولامین 2،3-دی اکسیژناز 1Differentiation - تفکیکHuman monocyte - تک سلولی انسانDichloroacetate - دی کلرواساتاتVascular endothelial growth factor A - فاکتور رشد اندوتلیال عروقی Alipopolysaccharide - لیپوپلی ساکاریدmatrix metallopeptidase 9 - ماتریکس متالوپپتیداز 9macrophage colony-stimulating factor - ماکروفاژ عامل کلونی تحریک کنندهcolony stimulating factor 1 - مستعد تحریک کلنی 1Glycolytic pathway - مسیر گلیکولیتیPyruvate dehydrogenase kinase - پیرووات دهیدروژناز کیناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
چکیده انگلیسی
Cellular metabolic state and individual metabolites have been reported to regulate the functional phenotype of immune cells. Cytokine production by regulatory and inflammatory macrophages is thought to mainly involve fatty acid oxidation and glycolysis, respectively, which fuel mitochondrial oxidative phosphorylation. However, the association between metabolic pathways and the acquisition of specific macrophage phenotypes remains unclear. This study assessed the relationship between glycolysis and the differentiation of regulatory macrophages. Human monocytes derived from peripheral blood were cultured in vitro in the presence of macrophage colony-stimulating factor to yield regulatory macrophages (M-MÏs). M-MÏs had a regulatory macrophage phenotype and produced substantial IL-10 following stimulation with lipopolysaccharide. To analyze the role of glycolysis, glycolysis inhibitors (2-deoxy-d-glucose or dichloroacetate) were added during M-MÏ differentiation. These cells cultured with glycolysis inhibitors produced significantly lower amounts of IL-10, but produced significantly higher amounts of IL-6 compared to M-MÏs differentiated without glycolysis inhibitors. Such phenotypic change of M-MÏs differentiated with glycolysis inhibitors was associated with the alteration of the gene expression pattern related to macrophage differentiation, such as CSF1, MMP9 and VEGFA. M-MÏs differentiated with glycolysis inhibitors seemed to retain plasticity to become IL-10 producing cells. Furthermore, increased level of pyruvate in culture medium was found to partially reverse the effects of glycolysis inhibitors on cytokine production of M-MÏs. These results indicate the importance of glycolytic pathway in macrophage differentiation to a regulatory phenotype, and pyruvate may be one of the key metabolites in this process.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Immunology Letters - Volume 176, August 2016, Pages 18-27
Journal: Immunology Letters - Volume 176, August 2016, Pages 18-27
نویسندگان
Hiroaki Suzuki, Tadakazu Hisamatsu, Sayako Chiba, Kiyoto Mori, Mina T. Kitazume, Katsuyoshi Shimamura, Nobuhiro Nakamoto, Katsuyoshi Matsuoka, Hirotoshi Ebinuma, Makoto Naganuma, Takanori Kanai,