کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6305194 | 1306682 | 2015 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A new, automated rapid fluorometric method for the detection of Escherichia coli in recreational waters
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
علوم زمین و سیارات
علوم زمین و سیاره ای (عمومی)
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![عکس صفحه اول مقاله: A new, automated rapid fluorometric method for the detection of Escherichia coli in recreational waters A new, automated rapid fluorometric method for the detection of Escherichia coli in recreational waters](/preview/png/6305194.png)
چکیده انگلیسی
Membrane filtration/culture techniques have been demonstrated to be reliable and broadly applicable for determination of fecal contamination in recreational waters. The time-consuming nature of culture techniques, however, is viewed as the major disadvantage of this type of analysis, and many authors have asserted the need for improved rapid-detection methods. In this study, we evaluated the performance of the ENDETEC⢠TECTA⢠B16, an automated fluorometry-based microbial detection system, by comparing its detection time and accuracy to those of two common culture-based methods, which are widely-used for recreational water quality monitoring in Canada. Our results demonstrated that Escherichia coli densities inferred by the TECTA⢠method were generally in agreement with those generated by standard culture methods (y = 1.19x + 0.002, R2 = 0.89) and under the current calibration regime, TECTA⢠tended to slightly overestimate E. coli densities. In addition, TECTA⢠was able to detect E. coli densities in exceedance of the Ontario Provincial Water Quality Objective for recreational waters in as little as 4 h (mean detection time = 7.03 h), representing a substantial improvement over traditional culture techniques. Our findings suggest that with improved calibration, TECTA⢠may provide a viable, cost-effective, rapid alternative to culture approaches for the detection of fecal contamination in recreational waters.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Great Lakes Research - Volume 41, Issue 1, March 2015, Pages 298-302
Journal: Journal of Great Lakes Research - Volume 41, Issue 1, March 2015, Pages 298-302
نویسندگان
Andrew J. Bramburger, R. Stephen Brown, Jennifer Haley, Jeffrey J. Ridal,