کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6309529 | 1618875 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Bisphenol A promotes dendritic morphogenesis of hippocampal neurons through estrogen receptor-mediated ERK1/2 signal pathway
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کلمات کلیدی
NR2BNMDA17β-E2N-methyl-d-aspartateRac1ECLPMSFF-actinPBSTCDC42FBSGPR30BPAN-methyl-d-aspartate receptor subunit 2BDABPBSRho ADMEM17beta-estradiol - 17 بتا استرادیولfilamentous actin - actin filamentousBSA - BSADMSO - DMSOERKs - ERK هاERK1/2 - ERK1 / 2MAPK - MAPKDulbecco’s modified Eagle medium - Modified Eagle اصلاح شده DulbeccoSDS–PAGE - SDS-PAGEbovine serum albumin - آلبومین سرم گاوenhanced chemiluminescence - بهبود شیمیایی لومنBisphenol A - بیسفنول ای، بیسفنول Astandard error of mean - خطای استاندارد میانگینCNS - دستگاه عصبی مرکزیdiaminobenzidine - دیامینو بنزیدینDimethyl sulfoxide - دیمتیل سولفواکسیدfetal bovine serum - سرم جنین گاوcentral nervous system - سیستم عصبی مرکزیPhosphate buffered saline - فسفات بافر شورPhenylmethanesulfonyl fluoride - فنیل متیل سولفونیل فلورایدMEK - مجاهدین خلقSEM - مدل معادلات ساختاری / میکروسکوپ الکترونی روبشیMorphogenesis - مورفوژنزHippocampal neurons - نورونهای هیپوکامپmitogen-activated protein kinase - پروتئین کیناز فعال با mitogenextracellular signal-regulated kinase 1/2 - کیناز 1/2 تنظیم سیگنال خارج سلولیEstrogen receptor - گیرنده استروژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Bisphenol A (BPA), an environmental endocrine disruptor, has attracted increasing attention to its adverse effects on brain developmental process. The previous study indicated that BPA rapidly increased motility and density of dendritic filopodia and enhanced the phosphorylation of N-methyl-d-aspartate (NMDA) receptor subunit NR2B in cultured hippocampal neurons within 30 min. The purpose of the present study was further to investigate the effects of BPA for 24 h on dendritic morphogenesis and the underlying mechanisms. After cultured for 5 d in vitro, the hippocampal neurons from 24 h-old rat were infected by AdV-EGFP to indicate time-lapse imaging of living neurons. The results demonstrated that the exposure of the cultured hippocampal neurons to BPA (10, 100 nM) or 17β-estradiol (17β-E2, 10 nM) for 24 h significantly promoted dendritic development, as evidenced by the increased total length of dendrite and the enhanced motility and density of dendritic filopodia. However, these changes were suppressed by an ERs antagonist, ICI182,780, a non-competitive NMDA receptor antagonist, MK-801, and a mitogen-activated ERK1/2-activating kinase (MEK1/2) inhibitor, U0126. Meanwhile, the increased F-actin (filamentous actin) induced by BPA (100 nM) was also completely eliminated by these blockers. Furthermore, the result of western blot analyses showed that, the exposure of the cultures to BPA or 17β-E2 for 24 h promoted the expression of Rac1/Cdc42 but inhibited that of RhoA, suggesting Rac1 (Ras related C3 botulinum toxinsubstrate 1)/Cdc42 (cell divisioncycle 42) and RhoA (Ras homologous A), the Rho family of small GTPases, were involved in BPA- or 17β-E2-induced changes in the dendritic morphogenesis of neurons. These BPA- or 17β-E2-induced effects were completely blocked by ICI182,780, and were partially suppressed by U0126. These results reveal that, similar to 17β-E2, BPA exerts its effects on dendritic morphogenesis by eliciting both nuclear actions and extranuclear-initiated actions that are integrated to influence the development of dendrite in hippocampal neurons.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemosphere - Volume 96, February 2014, Pages 129-137
Journal: Chemosphere - Volume 96, February 2014, Pages 129-137
نویسندگان
Xiaohong Xu, Yang Lu, Guangxia Zhang, Lei Chen, Dong Tian, Xiuying Shen, Yanling Yang, Fanni Dong,