Fish bile as a biomarker for metal exposure

► Biliary excretion is an alternative way to evaluate metals exposure in fish. ► Liver and bile trace elements were correlated by Artificial Neural Networks (ANN). ► Significant correlations were observed for some trace elements in bile and liver. ► The ANN shows show that metals in bile can be used instead of liver concentrations. ► Bile is proposed as being used instead of liver as a metal exposure bioindicator.