کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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6362882 | 1315698 | 2009 | 5 صفحه PDF | دانلود رایگان |

The hemolytic activity of the dinoflagellate Cochlodinium polykrikoides from BahÃa de La Paz, Gulf of California was investigated as part of the ichthyotoxic mechanism of this microalga. Two different kinds of erythrocytes, fish and human, were tested for the hemolytic assay. Since fatty acids have been associated with hemolytic activity in C. polykrikoides, the composition of fatty acids of this dinoflagellate was also analyzed. The concentration of C. polykrikoides causing 50% hemolysis (HE50) was 4.88 and 5.27 Ã 106 cells Lâ1, for fish and human erythrocytes, respectively. According to the standard curve of saponin, an equivalence between the hemolytic activity of saponin and the dinoflagellate concentration was found with 1 μg saponin mLâ1 equivalent to 1 Ã 106 cells Lâ1 of C. polykrikoides. The polyunsaturated fatty acids: hexadecaenoic (16:0), docosahexaenoic (22:6 n3) and octadecapentaenoic (18:5 n3) were found in an abundance of â¼62% of total fatty acids.
Journal: Marine Pollution Bulletin - Volume 58, Issue 9, September 2009, Pages 1401-1405