کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6490639 | 43376 | 2016 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Hyperforin production in Hypericum perforatum root cultures
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کلمات کلیدی
EPIMurashige-Skoog mediumDCHAESI–MS/MSLupulonesHyperforinsHypericum perforatumTRPC6IBAHypericinsAPIPVDFIAAHPLC-DADESIAUC - AUCESI–MS - ESI-MSindole-3-butyric acid - اسید Indole-3-Butyricindole-3-acetic acid - اسید Indole-3-استیکenhanced product ion scan - افزایش اسکن یون محصولDicyclohexylamine - دیسیکلو هگزیلامینelectrospray ionization–mass spectrometry - طیف سنجی جرم یونیزاسیون الکترو اسپریExtraction yield - عملکرد استخراجRoot cultures - فرهنگ ریشهReverse phase - مرحله معکوسarea under the curve - منطقه تحت منحنیActive Pharmaceutical Ingredient - مواد اولیه دارویی فعالelectrospray ionization - یونیزاسیون الکترو اسپری
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Extracts of the medicinal plant Hypericum perforatum are used to treat depression and skin irritation. A major API is hyperforin, characterized by sensitivity to light, oxygen and temperature. Total synthesis of hyperforin is challenging and its content in field-grown plants is variable. We have established in vitro cultures of auxin-induced roots, which are capable of producing hyperforin, as indicated by HPLC-DAD and ESI-MS analyses. The extraction yield and the productivity upon use of petroleum ether after solvent screening were â¼5Â mg/g DW and â¼50Â mg/L culture after six weeks of cultivation. The root cultures also contained secohyperforin and lupulones, which were not yet detected in intact plants. In contrast, they lacked another class of typical H. perforatum constituents, hypericins, as indicated by the analysis of methanolic extracts. Hyperforins and lupulones were stabilized and enriched as dicyclohexylammonium salts. Upon up-scaling of biomass production and downstream processing, H. perforatum root cultures may provide an alternative platform for the preparation of medicinal extracts and the isolation of APIs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 222, 20 March 2016, Pages 47-55
Journal: Journal of Biotechnology - Volume 222, 20 March 2016, Pages 47-55
نویسندگان
Mariam Gaid, Paul Haas, Till Beuerle, Stephan Scholl, Ludger Beerhues,