Efficient enzymatic synthesis of ampicillin by mutant Alcaligenes faecalis penicillin G acylase

- Protein engineering was performed on thermo-stable Alcaligenes faecalis penicillin G acylase.
- A screened mutant showed up to 800-fold increased catalytic efficiency for activated acyl donor.
- 95% of 6-APA could be converted to ampicillin in aqueous medium by mutant PGA.
- Process productivity of enzymatic synthesis of ampicillin was improved by more than 130 times.