کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6494849 | 1418372 | 2018 | 29 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Deciphering the growth pattern and phytohormonal content in Saskatoon berry (Amelanchier alnifolia) in response to in vitro cytokinin application
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کلمات کلیدی
PPFMeta-topolinDHZN6-IsopentenyladenineIPRMTRtZRiP9GESIDHZRcZRcZOGcis-zeatinDihydrozeatin-O-glucosideBA9GN6-benzyladeninemTOGIAAOTRMurashige and Skoog medium - Murashige و Skoog mediumPTr - PTRindole-3-acetic acid - اسید Indole-3-استیکOrganogenesis - اندام زایی، ارگانوژنزAuxins - اکسینBAR - بارtrans-Zeatin - ترانس اکتینdihydrozeatin - دی هیدروزیاتینMicropropagation - ریز ازدیادیcytokinin - سیتوکینینEndogenous cytokinins - سیتوکینین های اندوژنphotosynthetic photon flux density - فتوسنتز تراکم شار فوتونUltra-high performance liquid chromatography - کروماتوگرافی مایع با کارایی فوق العاده بالاkinetin - کینتین
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Deciphering the growth pattern and phytohormonal content in Saskatoon berry (Amelanchier alnifolia) in response to in vitro cytokinin application Deciphering the growth pattern and phytohormonal content in Saskatoon berry (Amelanchier alnifolia) in response to in vitro cytokinin application](/preview/png/6494849.png)
چکیده انگلیسی
Clonal propagation plays a critical integral role in the growth and success of a global multi-billion dollar horticulture industry through a constant supply of healthy stock plants. The supply chain depends on continuously improving the micropropagation process, thus, understanding the physiology of in vitro plants remains a core component. We evaluated the influence of exogenously applied cytokinins (CKs, N6-benzyladenineâ¯=â¯BA, isopentenyladenineâ¯=â¯iP, meta-topolinâ¯=â¯mT, 6-(3-hydroxybenzylamino)-9-(tetrahydropyran-2-yl)purineâ¯=â¯mTTHP) in Murashige and Skoog (MS)-supplemented media on organogenic response and accumulation of endogenous CK and indole-3-acetic acid (IAA) metabolites. The highest shoot proliferation (30 shoots/explant) was obtained with 20â¯Î¼MâmT treatment. However, the best quality regenerants were produced in 10â¯Î¼MâmT treatment. Rooting of Amelanchier alnifolia in vitro plantlets was observed at the lowest CK concentrations, with the highest root proliferation (3 roots/explant) in 1â¯Î¼M mTTHP regenerants. Similar to the organogenic response, high levels of endogenous bioactive CK metabolites (free bases, ribosides, and nucleotides) were detected in mT and mTTHP-derived regenerants. The level of O-glucosides was also comparatively high in these cultures. All CK-treated plants had high levels of endogenous free IAA compared to the control. This may suggest an influence of CKs on biosynthesis of IAA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: New Biotechnology - Volume 42, 25 May 2018, Pages 85-94
Journal: New Biotechnology - Volume 42, 25 May 2018, Pages 85-94
نویسندگان
Mack Moyo, Adeyemi O. Aremu, Lenka PlaÄková, Lucie PlÃhalová, AleÅ¡ PÄnÄÃk, OndÅej Novák, Jan Holub, Karel Doležal, Johannes Van Staden,