کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6494892 | 1418374 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Anti-inflammatory and anti-oxidant properties of laccase-synthesized phenolic-O-carboxymethyl chitosan hydrogels
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کلمات کلیدی
CmCSO-carboxymethyl chitosanDMEMMMP-1DPPHHNEMTLTEACFGF2MPO - DFOROS - ROSSinapyl alcohol - الکل سیناپیلHuman neutrophil elastase - انسانی نوتروفیل انسانیEugenol - اوژنولsin - بدونAnti-oxidant - ضد اکسید کنندهAnti-inflammatory - ضدالتهابTrolox equivalent antioxidant capacity - ظرفیت آنتی اکسیدانی معادل ترولوکسfibroblast growth factor 2 - عامل رشد فیبروبلاست 2Phenolics - فنولیکLaccase - لاکاس یا لاکازmatrix metalloproteinase - ماتریکس متالوپروتئینازmyeloperoxidase - میلوپراکسیداز Chitosan hydrogel - هیدروژل کیتوزانCatechol - کاتچولCaffeic acid - کافئیک اسیدChitosan - کیتوسان Reactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A bioactive O-carboxymethyl chitosan (CMCS) hydrogel crosslinked with natural phenolics with potential application in wound dressings was synthesized using a laccase from Myceliophthora thermophila (MTL). The highest degree of cross-linking (49.7%) was achieved with catechol. All the phenolic-CMCS hydrogels synthesized showed excellent anti-oxidant properties with a free radical scavenging activity up to 4-fold higher than in the absence of the phenolics, as quantified by the di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium (DPPH) assay. In addition, the hydrogels produced showed an anti-inflammatory effect as evidenced by the inhibition of enzymes [myeloperoxidase (MPO), matrix-metalloproteinase-1 (MMP-1) and human neutrophil elastase (HNE)] over-expressed in chronic wounds. Sinapyl-CMCS hydrogels showed an MMP-1 inhibition of 37%. Further, the phenolic-CMCS hydrogels did not affect the viability of the NIH 3T3 mouse fibroblast cell line and were also able to slowly release human fibroblast growth factor 2, reaching 48.3% over a period of 28Â days. This study thus shows the possibility of synthesizing multifunctional bioactive chitosan based hydrogels with anti-oxidant and anti-inflammatory properties using natural occurring phenolics as crosslinkers.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: New Biotechnology - Volume 40, Part B, 25 January 2018, Pages 236-244
Journal: New Biotechnology - Volume 40, Part B, 25 January 2018, Pages 236-244
نویسندگان
Daniela Huber, Adrianna Grzelak, Martina Baumann, Nicole Borth, Gerhard Schleining, Gibson S. Nyanhongo, Georg M. Guebitz,