A novel method for pullulanase immobilized onto magnetic chitosan/Fe3O4 composite nanoparticles by in situ preparation and evaluation of the enzyme stability

- Pullulanase was immobilized on magnetic nanoparticles prepared by in situ method.
- High activity retention was obtained via optimizing process condition.
- Immobilized pullulanase with good thermal and operational stability were obtained.
- Immobilized enzyme exhibited a broadened and more acidic pH profiles.