Effect of the binding of bivalent ion to the calcium-binding site responsible for the thermal stability of actinomycete mannanase: Potential use in production of functional mannooligosaccharides

- Bivalent ion binding to the calcium-binding site in mannanase enhanced thermal stability.
- Calcium-binding site deletion mutants did not bind calcium, manganese, and magnesium ions.
- Enhancement of thermal stability was related to the association constant Ka.
- Ka comprising negative enthalpy and low entropy was suitable for bivalent ion binding.