کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7231828 | 1470960 | 2015 | 42 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Replacing antibodies with aptamers in lateral flow immunoassay
ترجمه فارسی عنوان
جایگزینی آنتیبادی ها با آپتامرها در آزمایش ایمونولوژی جریان جانبی
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کلمات کلیدی
LFIAICAPOCTGNPOTASELEXLMWAFB1 - AFB 1cDNA - cDNADNA - DNA یا اسید دزوکسی ریبونوکلئیکImmunochromatographic assay - آزمایش ایمونو کروماتوگرافیStrip test - آزمون نوارAflatoxin B1 - آفلاتوکسین B1Aptamer - آپتامرهاdeoxyribose nucleic acid - اسید نوکلئیک deoxyriboseOchratoxin A - اکراتوکسین AImmunochromatography - ایمونو کروماتوگرافیLateral flow immunoassay - ایمونویسی جریان جانبیEnzyme-linked immunosorbent assay - تست الیزاELISA - تست الیزاPoint-of-care testing - تست نقطه مراقبتLateral flow - جریان جانبیNitrocellulose membrane - غشای نیتروسلولزGold nanoparticle - نانو ذرات طلاquantum dot - نقطه کوانتومیpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازAntibody - پادتَن یا آنتیبادی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Aptamers have been identified against various targets as a type of chemical or nucleic acid ligand by systematic evolution of ligands by exponential enrichment (SELEX) with high sensitivity and specificity. Aptamers show remarkable advantages over antibodies due to the nucleic acid nature and target-induced structure-switching properties and are widely used to design various fluorescent, electrochemical, or colorimetric biosensors. However, the practical applications of aptamer-based sensing and diagnostics are still lagging behind those of antibody-based tests. Lateral flow immunoassay (LFIA) represents a well established and appropriate technology among rapid assays because of its low cost and user-friendliness. The antibody-based platform is utilized to detect numerous targets, but it is always hampered by the antibody preparation time, antibody stability, and effect of modification on the antibody. Seeking alternatives to antibodies is an area of active research and is of tremendous importance. Aptamers are receiving increasing attention in lateral flow applications because of a number of important potential performance advantages. We speculate that aptamer-based LFIA may be one of the first platforms for commercial use of aptamer-based diagnosis. This review first gives an introduction to aptamer including the selection process SELEX with its focus on aptamer advantages over antibodies, and then depicts LFIA with its focus on aptamer opportunities in LFIA over antibodies. Furthermore, we summarize the recent advances in the development of aptamer-based lateral flow biosensing assays with the aim to provide a general guide for the design of aptamer-based lateral flow biosensing assays.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 71, 15 September 2015, Pages 230-242
Journal: Biosensors and Bioelectronics - Volume 71, 15 September 2015, Pages 230-242
نویسندگان
Ailiang Chen, Shuming Yang,